1AZV
FAMILIAL ALS MUTANT G37R CUZNSOD (HUMAN)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01 |
| Detector | RIGAKU |
| Spacegroup name | P 41 |
| Unit cell lengths | 67.200, 67.200, 83.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1spd |
| RMSD bond length | 0.011 |
| RMSD bond angle | 26.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.065 * | 0.383 * |
| Total number of observations | 101819 * | |
| Number of reflections | 29129 | |
| <I/σ(I)> | 17 | 1.8 |
| Completeness [%] | 99.1 | 99.6 |
| Redundancy | 3.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.5 | 20 MG/ML PROTEIN IN 10MM ACETATE PH 5.5 MIXED WITH PRECIPITATING SOLUTION OF 100 MM ACETATE PH 4.6, 2.0 M AMMONIUM SULFATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 1 | acetate | 5 (mM) | |
| 3 | 1 | 1 | sodium acetate | 50 (mM) | |
| 4 | 1 | 1 | ammonium sulfate | 1.0 (M) | |
| 5 | 1 | 2 | sodium acetate | 100 (mM) | |
| 6 | 1 | 2 | ammonium sulfate | 2.0 (M) |
