1A5Z
LACTATE DEHYDROGENASE FROM THERMOTOGA MARITIMA (TMLDH)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 105.480, 105.480, 187.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.910 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.100 | |
| Rmerge | 0.089 | |
| Total number of observations | 111661 * | |
| Number of reflections | 30205 | |
| Completeness [%] | 97.9 * | 96.5 * |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5.0 (mg/ml) | |
| 2 | 1 | drop | HEPES | 0.1 (M) | |
| 3 | 1 | reservoir | sodium acetate | 1.0 (M) | |
| 4 | 1 | reservoir | oxamate | 250 (mM) | |
| 5 | 1 | reservoir | NADH | 150 (mM) | |
| 6 | 1 | reservoir | fructose-1,6-bisphosphate | 150 (mM) | |
| 7 | 1 | reservoir | cadmium sulfate | 50 (mM) |
