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PDB: 24 results
1D3C
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BU of 1d3c by Molmil
MICHAELIS COMPLEX OF BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYLTRANSFERASE WITH GAMMA-CYCLODEXTRIN
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, CALCIUM ION, CYCLODEXTRIN GLYCOSYLTRANSFERASE, ...
Authors:Uitdehaag, J.C.M, Kalk, K.H, van der Veen, B.A, Dijkhuizen, L, Dijkstra, B.W.
Deposit date:1999-09-29
Release date:1999-12-22
Last modified:2023-08-09
Method:X-RAY DIFFRACTION (1.78 Å)
Cite:The cyclization mechanism of cyclodextrin glycosyltransferase (CGTase) as revealed by a gamma-cyclodextrin-CGTase complex at 1.8-A resolution.
J.Biol.Chem., 274, 1999
1CXL
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COMPLEX BETWEEN A COVALENT INTERMEDIATE AND BACILLUS CIRCULANS STRAIN 251 CGTASE E257Q
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, 4-deoxy-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, 4-deoxy-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ...
Authors:Uitdehaag, J.C.M, Dijkstra, B.W.
Deposit date:1999-02-27
Release date:1999-05-03
Last modified:2023-08-09
Method:X-RAY DIFFRACTION (1.81 Å)
Cite:X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family.
Nat.Struct.Biol., 6, 1999
1KCL
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BU of 1kcl by Molmil
Bacillus ciruclans strain 251 Cyclodextrin glycosyl transferase mutant G179L
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, CALCIUM ION, Cyclodextrin glycosyltransferase, ...
Authors:Rozeboom, H.J, Uitdehaag, J.C.M, Dijkstra, B.W.
Deposit date:2001-11-09
Release date:2002-01-16
Last modified:2023-08-16
Method:X-RAY DIFFRACTION (1.94 Å)
Cite:The remote substrate binding subsite -6 in cyclodextrin-glycosyltransferase controls the transferase activity of the enzyme via an induced-fit mechanism.
J.Biol.Chem., 277, 2002
1PJ9
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BU of 1pj9 by Molmil
Bacillus circulans strain 251 loop mutant 183-195
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, ACETIC ACID, CALCIUM ION, ...
Authors:Rozeboom, H.J, Dijkstra, B.W.
Deposit date:2003-06-02
Release date:2004-02-03
Last modified:2023-08-16
Method:X-RAY DIFFRACTION (2 Å)
Cite:Improved thermostability of bacillus circulans cyclodextrin glycosyltransferase by the introduction of a salt bridge
PROTEINS: STRUCT.,FUNCT.,GENET., 54, 2004
1OT1
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BU of 1ot1 by Molmil
Bacillus circulans strain 251 Cyclodextrin glycosyl transferase mutant D135A
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, ACETIC ACID, ...
Authors:Rozeboom, H.J, Dijkstra, B.W.
Deposit date:2003-03-21
Release date:2003-06-03
Last modified:2023-08-16
Method:X-RAY DIFFRACTION (2 Å)
Cite:The fully conserved Asp residue in Conserved sequence region I of the alpha-amylase Family is crucial for the Catalytic Site Architecture and Activity
Febs Lett., 541, 2003
1CDG
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BU of 1cdg by Molmil
NUCLEOTIDE SEQUENCE AND X-RAY STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 IN A MALTOSE-DEPENDENT CRYSTAL FORM
Descriptor: CALCIUM ION, CYCLODEXTRIN GLYCOSYL-TRANSFERASE, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose
Authors:Lawson, C.L, Van Montfort, R, Strokopytov, B.V, Kalk, K.H, Rozeboom, H.J, Dijkstra, B.W.
Deposit date:1993-08-02
Release date:1994-01-31
Last modified:2020-07-29
Method:X-RAY DIFFRACTION (2 Å)
Cite:Nucleotide sequence and X-ray structure of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 in a maltose-dependent crystal form.
J.Mol.Biol., 236, 1994
1EO5
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BU of 1eo5 by Molmil
Bacillus circulans strain 251 cyclodextrin glycosyltransferase in complex with maltoheptaose
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, CALCIUM ION, PROTEIN (CYCLODEXTRIN GLYCOSYLTRANSFERASE), ...
Authors:Uitdehaag, J.C.M, Dijkstra, B.W.
Deposit date:2000-03-22
Release date:2000-11-22
Last modified:2023-08-09
Method:X-RAY DIFFRACTION (2 Å)
Cite:Structures of maltohexaose and maltoheptaose bound at the donor sites of cyclodextrin glycosyltransferase give insight into the mechanisms of transglycosylation activity and cyclodextrin size specificity.
Biochemistry, 39, 2000
1CXK
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COMPLEX BETWEEN A MALTONONAOSE SUBSTRATE AND BACILLUS CIRCULANS STRAIN 251 CGTASE E257Q/D229N
Descriptor: CALCIUM ION, PROTEIN (CYCLODEXTRIN-GLYCOSYLTRANSFERASE), alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ...
Authors:Uitdehaag, J.C.M, Kalk, K.H, Dijkstra, B.W.
Deposit date:1999-02-24
Release date:1999-05-03
Last modified:2023-08-09
Method:X-RAY DIFFRACTION (2.09 Å)
Cite:X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family.
Nat.Struct.Biol., 6, 1999
1CXE
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BU of 1cxe by Molmil
COMPLEX OF CGTASE WITH MALTOTETRAOSE AT ROOM TEMPERATURE AND PH 9.1 BASED ON DIFFRACTION DATA OF A CRYSTAL SOAKED WITH ALPHA-CYCLODEXTRIN
Descriptor: CALCIUM ION, CYCLODEXTRIN GLYCOSYLTRANSFERASE, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ...
Authors:Knegtel, R.M.A, Strokopytov, B.V, Dijkstra, B.W.
Deposit date:1995-07-28
Release date:1995-12-15
Last modified:2020-07-29
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:Crystallographic studies of the interaction of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 with natural substrates and products.
J.Biol.Chem., 270, 1995
1CXF
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COMPLEX OF A (D229N/E257Q) DOUBLE MUTANT CGTASE FROM BACILLUS CIRCULANS STRAIN 251 WITH MALTOTETRAOSE AT 120 K AND PH 9.1 OBTAINED AFTER SOAKING THE CRYSTAL WITH ALPHA-CYCLODEXTRIN
Descriptor: CALCIUM ION, CYCLODEXTRIN GLYCOSYLTRANSFERASE, Cyclohexakis-(1-4)-(alpha-D-glucopyranose), ...
Authors:Knegtel, R.M.A, Strokopytov, B.V, Dijkstra, B.W.
Deposit date:1995-07-28
Release date:1995-12-15
Last modified:2020-07-29
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:Crystallographic studies of the interaction of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 with natural substrates and products.
J.Biol.Chem., 270, 1995
1OT2
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BU of 1ot2 by Molmil
Bacillus circulans strain 251 Cyclodextrin glycosyl transferase mutant D135N
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, ACETIC ACID, ...
Authors:Rozeboom, H.J, Dijkstra, B.W.
Deposit date:2003-03-21
Release date:2003-06-03
Last modified:2023-08-16
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:The fully conserved Asp residue in Conserved sequence region I of the alpha-amylase Family is crucial for the Catalytic Site Architecture and Activity
Febs Lett., 541, 2003
1TCM
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BU of 1tcm by Molmil
CYCLODEXTRIN GLYCOSYLTRANSFERASE W616A MUTANT FROM BACILLUS CIRCULANS STRAIN 251
Descriptor: CALCIUM ION, CYCLODEXTRIN GLYCOSYLTRANSFERASE
Authors:Knegtel, R.M.A, Dijkstra, B.W.
Deposit date:1996-10-07
Release date:1997-04-21
Last modified:2021-11-03
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:The raw starch binding domain of cyclodextrin glycosyltransferase from Bacillus circulans strain 251.
J.Biol.Chem., 271, 1996
1CXI
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BU of 1cxi by Molmil
WILD-TYPE CGTASE FROM BACILLUS CIRCULANS STRAIN 251 AT 120 K AND PH 7.55
Descriptor: CALCIUM ION, CYCLODEXTRIN GLYCOSYLTRANSFERASE, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose
Authors:Knegtel, R.M.A, Strokopytov, B.V, Dijkstra, B.W.
Deposit date:1995-07-31
Release date:1995-12-15
Last modified:2020-07-29
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Crystallographic studies of the interaction of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 with natural substrates and products.
J.Biol.Chem., 270, 1995
1PEZ
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BU of 1pez by Molmil
Bacillus circulans strain 251 mutant A230V
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, ACETIC ACID, ...
Authors:Rozeboom, H.J, Dijkstra, B.W.
Deposit date:2003-05-23
Release date:2003-10-28
Last modified:2023-08-16
Method:X-RAY DIFFRACTION (2.32 Å)
Cite:Conversion of Cyclodextrin Glycosyltransferase into a Starch Hydrolase by Directed Evolution: The Role of Alanine 230 in Acceptor Subsite +1
Biochemistry, 42, 2003
1DTU
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BU of 1dtu by Molmil
BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYLTRANSFERASE: A MUTANT Y89D/S146P COMPLEXED TO AN HEXASACCHARIDE INHIBITOR
Descriptor: 1-AMINO-2,3-DIHYDROXY-5-HYDROXYMETHYL CYCLOHEX-5-ENE, CALCIUM ION, PROTEIN (CYCLODEXTRIN GLYCOSYLTRANSFERASE), ...
Authors:Uitdehaag, J.C.M, Kalk, K.H, Dijkstra, B.W.
Deposit date:2000-01-13
Release date:2000-03-06
Last modified:2023-08-09
Method:X-RAY DIFFRACTION (2.4 Å)
Cite:Rational design of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 to increase alpha-cyclodextrin production.
J.Mol.Biol., 296, 2000
1CXH
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BU of 1cxh by Molmil
COMPLEX OF CGTASE WITH MALTOTETRAOSE AT ROOM TEMPERATURE AND PH 9.6 BASED ON DIFFRACTION DATA OF A CRYSTAL SOAKED WITH MALTOHEPTAOSE
Descriptor: CALCIUM ION, CYCLODEXTRIN GLYCOSYLTRANSFERASE, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ...
Authors:Knegtel, R.M.A, Strokopytov, B.V, Dijkstra, B.W.
Deposit date:1995-07-31
Release date:1995-12-15
Last modified:2020-07-29
Method:X-RAY DIFFRACTION (2.41 Å)
Cite:Crystallographic studies of the interaction of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 with natural substrates and products.
J.Biol.Chem., 270, 1995
1KCK
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Bacillus circulans strain 251 Cyclodextrin glycosyl transferase mutant N193G
Descriptor: 1-AMINO-2,3-DIHYDROXY-5-HYDROXYMETHYL CYCLOHEX-5-ENE, CALCIUM ION, CYCLODEXTRIN GLYCOSYLTRANSFERASE, ...
Authors:Rozeboom, H.J, Uitdehaag, J.C.M, Dijkstra, B.W.
Deposit date:2001-11-09
Release date:2002-01-16
Last modified:2023-08-16
Method:X-RAY DIFFRACTION (2.43 Å)
Cite:The remote substrate binding subsite -6 in cyclodextrin-glycosyltransferase controls the transferase activity of the enzyme via an induced-fit mechanism.
J.Biol.Chem., 277, 2002
1EO7
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BU of 1eo7 by Molmil
BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYLTRANSFERASE IN COMPLEX WITH MALTOHEXAOSE
Descriptor: CALCIUM ION, PROTEIN (CYCLODEXTRIN GLYCOSYLTRANSFERASE), alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ...
Authors:Uitdehaag, J.C.M, Dijkstra, B.W.
Deposit date:2000-03-22
Release date:2000-11-22
Last modified:2023-08-09
Method:X-RAY DIFFRACTION (2.48 Å)
Cite:Structures of maltohexaose and maltoheptaose bound at the donor sites of cyclodextrin glycosyltransferase give insight into the mechanisms of transglycosylation activity and cyclodextrin size specificity.
Biochemistry, 39, 2000
2CXG
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CYCLODEXTRIN GLYCOSYLTRANSFERASE COMPLEXED TO THE INHIBITOR ACARBOSE
Descriptor: 6-AMINO-4-HYDROXYMETHYL-CYCLOHEX-4-ENE-1,2,3-TRIOL, CALCIUM ION, CYCLODEXTRIN GLYCOSYLTRANSFERASE, ...
Authors:Strokopytov, B.V, Uitdehaag, J.C.M, Ruiterkamp, R, Dijkstra, B.W.
Deposit date:1998-05-08
Release date:1998-10-14
Last modified:2023-08-09
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:X-ray structure of cyclodextrin glycosyltransferase complexed with acarbose. Implications for the catalytic mechanism of glycosidases.
Biochemistry, 34, 1995
1CGV
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SITE DIRECTED MUTATIONS OF THE ACTIVE SITE RESIDUE TYROSINE 195 OF CYCLODEXTRIN GLYCOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 AFFECTING ACTIVITY AND PRODUCT SPECIFICITY
Descriptor: CALCIUM ION, CYCLOMALTODEXTRIN GLUCANOTRANSFERASE, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose
Authors:Strokopytov, B.V, Dijkstra, B.W.
Deposit date:1994-08-05
Release date:1995-02-27
Last modified:2020-07-29
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Site-directed mutations in tyrosine 195 of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 affect activity and product specificity.
Biochemistry, 34, 1995
1CGX
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SITE DIRECTED MUTATIONS OF THE ACTIVE SITE RESIDUE TYROSINE 195 OF CYCLODEXTRIN GLYXOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 AFFECTING ACTIVITY AND PRODUCT SPECIFICITY
Descriptor: CALCIUM ION, CYCLOMALTODEXTRIN GLUCANOTRANSFERASE, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose
Authors:Strokopytov, B.V, Dijkstra, B.W.
Deposit date:1994-08-05
Release date:1995-02-07
Last modified:2020-07-29
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Site-directed mutations in tyrosine 195 of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 affect activity and product specificity.
Biochemistry, 34, 1995
1CGW
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BU of 1cgw by Molmil
SITE DIRECTED MUTATIONS OF THE ACTIVE SITE RESIDUE TYROSINE 195 OF CYCLODEXTRIN GLYCOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 AFFECTING ACTIVITY AND PRODUCT SPECIFICITY
Descriptor: CALCIUM ION, CYCLOMALTODEXTRIN GLUCANOTRANSFERASE, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose
Authors:Strokopytov, B.V, Dijkstra, B.W.
Deposit date:1994-08-05
Release date:1995-02-07
Last modified:2020-07-29
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Site-directed mutations in tyrosine 195 of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 affect activity and product specificity.
Biochemistry, 34, 1995
1CGY
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SITE DIRECTED MUTATIONS OF THE ACTIVE SITE RESIDUE TYROSINE 195 OF CYCLODEXTRIN GLYXOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 AFFECTING ACTIVITY AND PRODUCT SPECIFICITY
Descriptor: CALCIUM ION, CYCLOMALTODEXTRIN GLUCANOTRANSFERASE, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose
Authors:Strokopytov, B.V, Dijkstra, B.W.
Deposit date:1994-08-05
Release date:1995-02-07
Last modified:2020-07-29
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Site-directed mutations in tyrosine 195 of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 affect activity and product specificity.
Biochemistry, 34, 1995
2DIJ
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BU of 2dij by Molmil
COMPLEX OF A Y195F MUTANT CGTASE FROM B. CIRCULANS STRAIN 251 COMPLEXED WITH A MALTONONAOSE INHIBITOR AT PH 9.8 OBTAINED AFTER SOAKING THE CRYSTAL WITH ACARBOSE AND MALTOHEXAOSE
Descriptor: 1-AMINO-2,3-DIHYDROXY-5-HYDROXYMETHYL CYCLOHEX-5-ENE, CALCIUM ION, CYCLODEXTRIN GLYCOSYLTRANSFERASE, ...
Authors:Strokopytov, B.V, Knegtel, R.M.A, Uitdehaag, J.C.M, Dijkstra, B.W.
Deposit date:1998-05-27
Release date:1998-12-09
Last modified:2023-08-09
Method:X-RAY DIFFRACTION (2.6 Å)
Cite:Structure of cyclodextrin glycosyltransferase complexed with a maltononaose inhibitor at 2.6 angstrom resolution. Implications for product specificity.
Biochemistry, 35, 1996

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