4YVB
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![BU of 4yvb by Molmil](/molmil-images/mine/4yvb) | Structure of D128N streptavidin | Descriptor: | BIOTIN, Streptavidin | Authors: | Baugh, L, Le Trong, I, Stayton, P.S, Stenkamp, R.E, Lybrand, T.P. | Deposit date: | 2015-03-19 | Release date: | 2016-03-23 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (1.351 Å) | Cite: | A Streptavidin Binding Site Mutation Yields an Unexpected Result To Be Published
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1DF8
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![BU of 1df8 by Molmil](/molmil-images/mine/1df8) | S45A MUTANT OF STREPTAVIDIN IN COMPLEX WITH BIOTIN | Descriptor: | BIOTIN, PROTEIN (STREPTAVIDIN) | Authors: | Hyre, D.E, Le Trong, I, Freitag, S, Stenkamp, R.E, Stayton, P.S. | Deposit date: | 1999-11-18 | Release date: | 2000-09-20 | Last modified: | 2023-08-09 | Method: | X-RAY DIFFRACTION (1.51 Å) | Cite: | Ser45 plays an important role in managing both the equilibrium and transition state energetics of the streptavidin-biotin system. Protein Sci., 9, 2000
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3MG5
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![BU of 3mg5 by Molmil](/molmil-images/mine/3mg5) | Core-streptavidin mutant F130L in complex with biotin | Descriptor: | BIOTIN, GLYCEROL, Streptavidin | Authors: | Le Trong, I, Baugh, L, Stayton, P.S, Lybrand, T.P, Stenkamp, R.E. | Deposit date: | 2010-04-05 | Release date: | 2010-05-26 | Last modified: | 2023-09-06 | Method: | X-RAY DIFFRACTION (1.3 Å) | Cite: | A distal point mutation in the streptavidin-biotin complex preserves structure but diminishes binding affinity: experimental evidence of electronic polarization effects? Biochemistry, 49, 2010
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4GD9
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![BU of 4gd9 by Molmil](/molmil-images/mine/4gd9) | Circular Permuted Streptavidin N49/G48 | Descriptor: | BIOTIN, SULFATE ION, Streptavidin | Authors: | Le Trong, I, Chu, V, Xing, Y, Lybrand, T.P, Stayton, P.S, Stenkamp, R.E. | Deposit date: | 2012-07-31 | Release date: | 2013-06-05 | Last modified: | 2023-09-13 | Method: | X-RAY DIFFRACTION (1.5 Å) | Cite: | Structural consequences of cutting a binding loop: two circularly permuted variants of streptavidin. Acta Crystallogr.,Sect.D, 69, 2013
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4GDA
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![BU of 4gda by Molmil](/molmil-images/mine/4gda) | Circular Permuted Streptavidin A50/N49 | Descriptor: | BIOTIN, ETHANOL, GLYCEROL, ... | Authors: | Le Trong, I, Chu, V, Xing, Y, Lybrand, T.P, Stayton, P.S, Stenkamp, R.E. | Deposit date: | 2012-07-31 | Release date: | 2013-06-05 | Last modified: | 2024-02-28 | Method: | X-RAY DIFFRACTION (1 Å) | Cite: | Structural consequences of cutting a binding loop: two circularly permuted variants of streptavidin. Acta Crystallogr.,Sect.D, 69, 2013
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3RY1
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![BU of 3ry1 by Molmil](/molmil-images/mine/3ry1) | Wild-type core streptavidin at atomic resolution | Descriptor: | (4R)-2-METHYLPENTANE-2,4-DIOL, (4S)-2-METHYL-2,4-PENTANEDIOL, Streptavidin | Authors: | Stenkamp, R.E, Wang, Z, Le Trong, I, Stayton, P.S, Lybrand, T.P. | Deposit date: | 2011-05-10 | Release date: | 2011-08-24 | Last modified: | 2024-02-28 | Method: | X-RAY DIFFRACTION (1.03 Å) | Cite: | Streptavidin and its biotin complex at atomic resolution. Acta Crystallogr.,Sect.D, 67, 2011
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3T6F
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![BU of 3t6f by Molmil](/molmil-images/mine/3t6f) | Biotin complex of Y54F core streptavidin | Descriptor: | BIOTIN, BIOTIN-D-SULFOXIDE, GLYCEROL, ... | Authors: | Baugh, L, Le Trong, I, Cerutti, D.S, Mehta, N, Gulich, S, Stayton, P.S, Stenkamp, R.E, Lybrand, T.P. | Deposit date: | 2011-07-28 | Release date: | 2011-12-21 | Last modified: | 2023-09-13 | Method: | X-RAY DIFFRACTION (1.22 Å) | Cite: | Second-Contact Shell Mutation Diminishes Streptavidin-Biotin Binding Affinity through Transmitted Effects on Equilibrium Dynamics. Biochemistry, 51, 2012
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3T6L
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![BU of 3t6l by Molmil](/molmil-images/mine/3t6l) | Y54F mutant of core streptavidin | Descriptor: | 1,2-ETHANEDIOL, CHLORIDE ION, Streptavidin | Authors: | Baugh, L, Le Trong, I, Stayton, P.S, Stenkamp, R.E, Lybrand, T.P. | Deposit date: | 2011-07-28 | Release date: | 2011-12-21 | Last modified: | 2023-09-13 | Method: | X-RAY DIFFRACTION (1.3 Å) | Cite: | Second-Contact Shell Mutation Diminishes Streptavidin-Biotin Binding Affinity through Transmitted Effects on Equilibrium Dynamics. Biochemistry, 51, 2012
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3RY2
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![BU of 3ry2 by Molmil](/molmil-images/mine/3ry2) | Wild-type core streptavidin-biotin complex at atomic resolution | Descriptor: | BIOTIN, GLYCEROL, Streptavidin | Authors: | Stenkamp, R.E, Le Trong, I, Stayton, P.S, Lybrand, T.P. | Deposit date: | 2011-05-10 | Release date: | 2011-08-24 | Last modified: | 2023-09-13 | Method: | X-RAY DIFFRACTION (0.95 Å) | Cite: | Streptavidin and its biotin complex at atomic resolution. Acta Crystallogr.,Sect.D, 67, 2011
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1MM9
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![BU of 1mm9 by Molmil](/molmil-images/mine/1mm9) | Streptavidin Mutant with Insertion of Fibronectin Hexapeptide, including RGD | Descriptor: | (4R)-2-METHYLPENTANE-2,4-DIOL, Streptavidin | Authors: | Le Trong, I, McDevitt, T.C, Nelson, K.E, Stayton, P.S, Stenkamp, R.E. | Deposit date: | 2002-09-03 | Release date: | 2003-05-06 | Last modified: | 2024-02-14 | Method: | X-RAY DIFFRACTION (1.66 Å) | Cite: | Structural characterization and comparison of RGD cell-adhesion recognition sites engineered into streptavidin. Acta Crystallogr.,Sect.D, 59, 2003
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1MOY
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![BU of 1moy by Molmil](/molmil-images/mine/1moy) | Streptavidin Mutant with Osteopontin Hexapeptide Insertion Including RGD | Descriptor: | (4R)-2-METHYLPENTANE-2,4-DIOL, Streptavidin | Authors: | Le Trong, I, McDevitt, T.C, Nelson, K.E, Stayton, P.S, Stenkamp, R.E. | Deposit date: | 2002-09-10 | Release date: | 2003-05-06 | Last modified: | 2024-02-14 | Method: | X-RAY DIFFRACTION (1.55 Å) | Cite: | Structural characterization and comparison of RGD cell-adhesion recognition sites engineered into streptavidin. Acta Crystallogr.,Sect.D, 59, 2003
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1MK5
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![BU of 1mk5 by Molmil](/molmil-images/mine/1mk5) | Wildtype Core-Streptavidin with Biotin at 1.4A. | Descriptor: | BIOTIN, Streptavidin | Authors: | Hyre, D.E, Le Trong, I, Merritt, E.A, Green, N.M, Stenkamp, R.E, Stayton, P.S. | Deposit date: | 2002-08-28 | Release date: | 2003-09-09 | Last modified: | 2024-02-14 | Method: | X-RAY DIFFRACTION (1.4 Å) | Cite: | Cooperative hydrogen bond interactions in the streptavidin-biotin system Protein Sci., 15, 2006
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1MEP
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![BU of 1mep by Molmil](/molmil-images/mine/1mep) | Crystal Structure of Streptavidin Double Mutant S45A/D128A with Biotin: Cooperative Hydrogen-Bond Interactions in the Streptavidin-Biotin System. | Descriptor: | BIOTIN, Streptavidin | Authors: | Hyre, D.E, Le Trong, I, Merritt, E.A, Stenkamp, R.E, Green, N.M, Stayton, P.S. | Deposit date: | 2002-08-08 | Release date: | 2003-09-02 | Last modified: | 2024-02-14 | Method: | X-RAY DIFFRACTION (1.65 Å) | Cite: | Cooperative hydrogen bond interactions in the streptavidin-biotin system Protein Sci., 15, 2006
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1N43
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![BU of 1n43 by Molmil](/molmil-images/mine/1n43) | Streptavidin Mutant N23A with biotin at 1.89A | Descriptor: | BIOTIN, Streptavidin | Authors: | Le Trong, I, Freitag, S, Klumb, L.A, Chu, V, Stayton, P.S, Stenkamp, R.E. | Deposit date: | 2002-10-30 | Release date: | 2003-09-02 | Last modified: | 2024-02-14 | Method: | X-RAY DIFFRACTION (1.89 Å) | Cite: | Structural studies of hydrogen bonds in the high-affinity streptavidin-biotin complex: mutations of amino acids interacting with the ureido oxygen of biotin. Acta Crystallogr.,Sect.D, 59, 2003
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1N7Y
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![BU of 1n7y by Molmil](/molmil-images/mine/1n7y) | STREPTAVIDIN MUTANT N23E AT 1.96A | Descriptor: | Streptavidin | Authors: | Le Trong, I, Freitag, S, Klumb, L.A, Chu, V, Stayton, P.S, Stenkamp, R.E. | Deposit date: | 2002-11-18 | Release date: | 2003-09-02 | Last modified: | 2024-02-14 | Method: | X-RAY DIFFRACTION (1.96 Å) | Cite: | Structural studies of hydrogen bonds in the high-affinity streptavidin-biotin complex: mutations of amino acids interacting with the ureido oxygen of biotin. Acta Crystallogr.,Sect.D, 59, 2003
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1N9M
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![BU of 1n9m by Molmil](/molmil-images/mine/1n9m) | Streptavidin Mutant S27A with Biotin at 1.6A Resolution | Descriptor: | BIOTIN, Streptavidin | Authors: | Le Trong, I, Freitag, S, Klumb, L.A, Chu, V, Stayton, P.S, Stenkamp, R.E. | Deposit date: | 2002-11-25 | Release date: | 2003-09-02 | Last modified: | 2024-02-14 | Method: | X-RAY DIFFRACTION (1.6 Å) | Cite: | Structural studies of hydrogen bonds in the high-affinity streptavidin-biotin complex: mutations of amino acids interacting with the ureido oxygen of biotin. Acta Crystallogr.,Sect.D, 59, 2003
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1N9Y
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![BU of 1n9y by Molmil](/molmil-images/mine/1n9y) | Streptavidin Mutant S27A at 1.5A Resolution | Descriptor: | (4R)-2-METHYLPENTANE-2,4-DIOL, (4S)-2-METHYL-2,4-PENTANEDIOL, Streptavidin | Authors: | Le Trong, I, Freitag, S, Klumb, L.A, Chu, V, Stayton, P.S, Stenkamp, R.E. | Deposit date: | 2002-11-26 | Release date: | 2003-09-02 | Last modified: | 2023-08-16 | Method: | X-RAY DIFFRACTION (1.53 Å) | Cite: | Structural studies of hydrogen bonds in the high-affinity streptavidin-biotin complex: mutations of amino acids interacting with the ureido oxygen of biotin. Acta Crystallogr.,Sect.D, 59, 2003
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1N4J
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![BU of 1n4j by Molmil](/molmil-images/mine/1n4j) | STREPTAVIDIN MUTANT N23A AT 2.18A | Descriptor: | Streptavidin | Authors: | Le Trong, I, Freitag, S, Klumb, L.A, Chu, V, Stayton, P.S, Stenkamp, R.E. | Deposit date: | 2002-10-31 | Release date: | 2003-09-02 | Last modified: | 2024-02-14 | Method: | X-RAY DIFFRACTION (2.18 Å) | Cite: | Structural studies of hydrogen bonds in the high-affinity streptavidin-biotin complex: mutations of amino acids interacting with the ureido oxygen of biotin. Acta Crystallogr.,Sect.D, 59, 2003
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1NC9
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![BU of 1nc9 by Molmil](/molmil-images/mine/1nc9) | STREPTAVIDIN MUTANT Y43A WITH IMINOBIOTIN AT 1.8A RESOLUTION | Descriptor: | 2-IMINOBIOTIN, Streptavidin | Authors: | Le Trong, I, Freitag, S, Klumb, L.A, Chu, V, Stayton, P.S, Stenkamp, R.E. | Deposit date: | 2002-12-05 | Release date: | 2003-09-02 | Last modified: | 2023-08-16 | Method: | X-RAY DIFFRACTION (1.8 Å) | Cite: | Structural studies of hydrogen bonds in the high-affinity streptavidin-biotin complex: mutations of amino acids interacting with the ureido oxygen of biotin. Acta Crystallogr.,Sect.D, 59, 2003
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1NDJ
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![BU of 1ndj by Molmil](/molmil-images/mine/1ndj) | Streptavidin Mutant Y43F with Biotin at 1.81A Resolution | Descriptor: | BIOTIN, Streptavidin | Authors: | Le Trong, I, Freitag, S, Klumb, L.A, Chu, V, Stayton, P.S, Stenkamp, R.E. | Deposit date: | 2002-12-09 | Release date: | 2003-09-02 | Last modified: | 2023-08-16 | Method: | X-RAY DIFFRACTION (1.81 Å) | Cite: | Structural studies of hydrogen bonds in the high-affinity streptavidin-biotin complex: mutations of amino acids interacting with the ureido oxygen of biotin. Acta Crystallogr.,Sect.D, 59, 2003
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1NBX
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![BU of 1nbx by Molmil](/molmil-images/mine/1nbx) | Streptavidin Mutant Y43A at 1.70A Resolution | Descriptor: | (4R)-2-METHYLPENTANE-2,4-DIOL, Streptavidin | Authors: | Le Trong, I, Freitag, S, Klumb, L.A, Chu, V, Stayton, P.S, Stenkamp, R.E. | Deposit date: | 2002-12-04 | Release date: | 2003-09-02 | Last modified: | 2023-08-16 | Method: | X-RAY DIFFRACTION (1.7 Å) | Cite: | Structural studies of hydrogen bonds in the high-affinity streptavidin-biotin complex: mutations of amino acids interacting with the ureido oxygen of biotin. Acta Crystallogr.,Sect.D, 59, 2003
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1SWK
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![BU of 1swk by Molmil](/molmil-images/mine/1swk) | CORE-STREPTAVIDIN MUTANT W79F IN COMPLEX WITH BIOTIN AT PH 4.5 | Descriptor: | BIOTIN, CORE-STREPTAVIDIN, EPI-BIOTIN | Authors: | Freitag, S, Le Trong, I, Chilkoti, A, Klumb, L.A, Stayton, P.S, Stenkamp, R.E. | Deposit date: | 1998-01-27 | Release date: | 1999-02-09 | Last modified: | 2024-05-22 | Method: | X-RAY DIFFRACTION (2 Å) | Cite: | Structural studies of binding site tryptophan mutants in the high-affinity streptavidin-biotin complex. J.Mol.Biol., 279, 1998
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1SWH
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![BU of 1swh by Molmil](/molmil-images/mine/1swh) | CORE-STREPTAVIDIN MUTANT W79F AT PH 4.5 | Descriptor: | CORE-STREPTAVIDIN | Authors: | Freitag, S, Le Trong, I, Chilkoti, A, Klumb, L.A, Stayton, P.S, Stenkamp, R.E. | Deposit date: | 1998-01-27 | Release date: | 1999-02-09 | Last modified: | 2024-05-22 | Method: | X-RAY DIFFRACTION (1.7 Å) | Cite: | Structural studies of binding site tryptophan mutants in the high-affinity streptavidin-biotin complex. J.Mol.Biol., 279, 1998
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1SWR
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![BU of 1swr by Molmil](/molmil-images/mine/1swr) | CORE-STREPTAVIDIN MUTANT W120A IN COMPLEX WITH BIOTIN AT PH 7.5 | Descriptor: | BIOTIN, CORE-STREPTAVIDIN | Authors: | Freitag, S, Le Trong, I, Chilkoti, A, Klumb, L.A, Stayton, P.S, Stenkamp, R.E. | Deposit date: | 1998-01-27 | Release date: | 1999-02-09 | Last modified: | 2024-05-22 | Method: | X-RAY DIFFRACTION (1.9 Å) | Cite: | Structural studies of binding site tryptophan mutants in the high-affinity streptavidin-biotin complex. J.Mol.Biol., 279, 1998
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1SWN
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![BU of 1swn by Molmil](/molmil-images/mine/1swn) | CORE-STREPTAVIDIN MUTANT W108F IN COMPLEX WITH BIOTIN AT PH 7.0 | Descriptor: | BIOTIN, CORE-STREPTAVIDIN | Authors: | Freitag, S, Le Trong, I, Chilkoti, A, Klumb, L.A, Stayton, P.S, Stenkamp, R.E. | Deposit date: | 1998-01-27 | Release date: | 1999-02-09 | Last modified: | 2024-05-22 | Method: | X-RAY DIFFRACTION (2.2 Å) | Cite: | Structural studies of binding site tryptophan mutants in the high-affinity streptavidin-biotin complex. J.Mol.Biol., 279, 1998
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