8IC6
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![BU of 8ic6 by Molmil](/molmil-images/mine/8ic6) | exo-beta-D-arabinanase ExoMA2 from Microbacterium arabinogalactanolyticum in complex with Tris | Descriptor: | (4S)-2-METHYL-2,4-PENTANEDIOL, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, CHLORIDE ION, ... | Authors: | Fukushima, R, Kashima, T, Ishiwata, A, Fujita, K, Fushinobu, S. | Deposit date: | 2023-02-10 | Release date: | 2023-08-16 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (1.75 Å) | Cite: | Identification and characterization of endo-alpha-, exo-alpha-, and exo-beta-D-arabinofuranosidases degrading lipoarabinomannan and arabinogalactan of mycobacteria. Nat Commun, 14, 2023
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1KWG
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![BU of 1kwg by Molmil](/molmil-images/mine/1kwg) | Crystal structure of Thermus thermophilus A4 beta-galactosidase | Descriptor: | (4S)-2-METHYL-2,4-PENTANEDIOL, ACETATE ION, BETA-GALACTOSIDASE, ... | Authors: | Hidaka, M, Fushinobu, S, Ohtsu, N, Motoshima, H, Matsuzawa, H, Shoun, H, Wakagi, T. | Deposit date: | 2002-01-29 | Release date: | 2002-09-18 | Last modified: | 2024-03-13 | Method: | X-RAY DIFFRACTION (1.6 Å) | Cite: | Trimeric Crystal Structure of the Glycoside Hydrolase Family 42 beta-Galactosidase from Thermus thermophilus A4 and the Structure of its Complex with Galactose J.MOL.BIOL., 322, 2002
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5B1N
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![BU of 5b1n by Molmil](/molmil-images/mine/5b1n) | DHp domain structure of EnvZ from Escherichia coli | Descriptor: | Osmolarity sensor protein EnvZ | Authors: | Okajima, T, Eguchi, Y, Tochio, N, Inukai, Y, Shimizu, R, Ueda, S, Shinya, S, Kigawa, T, Fukamizo, T, Igarashi, M, Utsumi, R. | Deposit date: | 2015-12-09 | Release date: | 2016-12-14 | Last modified: | 2023-11-08 | Method: | X-RAY DIFFRACTION (1.33 Å) | Cite: | Angucycline antibiotic waldiomycin recognizes common structural motif conserved in bacterial histidine kinases J. Antibiot., 70, 2017
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5AX7
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![BU of 5ax7 by Molmil](/molmil-images/mine/5ax7) | yeast pyruvyltransferase Pvg1p | Descriptor: | Pyruvyl transferase 1, ZINC ION | Authors: | Kanekiyo, M, Yoritsune, K, Yoshinaga, S, Higuchi, Y, Takegawa, K, Kakuta, Y. | Deposit date: | 2015-07-16 | Release date: | 2016-06-08 | Last modified: | 2020-02-26 | Method: | X-RAY DIFFRACTION (2.46 Å) | Cite: | A rationally engineered yeast pyruvyltransferase Pvg1p introduces sialylation-like properties in neo-human-type complex oligosaccharide Sci Rep, 6, 2016
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3X2N
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![BU of 3x2n by Molmil](/molmil-images/mine/3x2n) | Proton relay pathway in inverting cellulase | Descriptor: | Endoglucanase V-like protein, SULFATE ION | Authors: | Nakamura, A, Ishida, T, Fushinobu, S, Igarashi, K, Samejima, M. | Deposit date: | 2014-12-22 | Release date: | 2015-10-14 | Last modified: | 2019-12-18 | Method: | X-RAY DIFFRACTION (1.2 Å) | Cite: | "Newton's cradle" proton relay with amide-imidic acid tautomerization in inverting cellulase visualized by neutron crystallography. Sci Adv, 1, 2015
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7DIF
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![BU of 7dif by Molmil](/molmil-images/mine/7dif) | GH127 beta-L-arabinofuranosidase HypBA1 covalently complexed with beta-L-arabinofuranose-configured cyclophellitol at 1.75-angstrom resolution | Descriptor: | (1S,2S,3R,4R)-3-(hydroxymethyl)cyclopentane-1,2,4-triol, Non-reducing end beta-L-arabinofuranosidase, POTASSIUM ION, ... | Authors: | Amaki, S, McGregor, N.G.S, Arakawa, T, Yamada, C, Borlandelli, V, Overkleeft, H.S, Davies, G.J, Fushinobu, S. | Deposit date: | 2020-11-19 | Release date: | 2021-01-27 | Last modified: | 2023-11-29 | Method: | X-RAY DIFFRACTION (1.75 Å) | Cite: | Cysteine Nucleophiles in Glycosidase Catalysis: Application of a Covalent beta-l-Arabinofuranosidase Inhibitor. Angew.Chem.Int.Ed.Engl., 60, 2021
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6KPL
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![BU of 6kpl by Molmil](/molmil-images/mine/6kpl) | Crystal Structure of endo-beta-N-acetylglucosaminidase from Cordyceps militaris in apo form | Descriptor: | Chitinase, DI(HYDROXYETHYL)ETHER, TRIETHYLENE GLYCOL | Authors: | Seki, H, Arakawa, T, Yamada, C, Takegawa, K, Fushinobu, S. | Deposit date: | 2019-08-15 | Release date: | 2019-10-02 | Last modified: | 2019-11-20 | Method: | X-RAY DIFFRACTION (1.75 Å) | Cite: | Structural basis for the specific cleavage of core-fucosylatedN-glycans by endo-beta-N-acetylglucosaminidase from the fungusCordyceps militaris. J.Biol.Chem., 294, 2019
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5B46
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![BU of 5b46 by Molmil](/molmil-images/mine/5b46) | 2-Oxoacid:Ferredoxin Oxidoreductase 2 from Sulfolobus tokodai - ligand free form | Descriptor: | 2-oxoacid--ferredoxin oxidoreductase alpha subunit, 2-oxoacid--ferredoxin oxidoreductase beta subunit, IRON/SULFUR CLUSTER, ... | Authors: | Yan, Z, Maruyama, A, Arakawa, T, Fushinobu, S, Wakagi, T. | Deposit date: | 2016-04-01 | Release date: | 2016-09-28 | Last modified: | 2024-03-20 | Method: | X-RAY DIFFRACTION (2.1 Å) | Cite: | Crystal structures of archaeal 2-oxoacid:ferredoxin oxidoreductases from Sulfolobus tokodaii Sci Rep, 6, 2016
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5B47
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![BU of 5b47 by Molmil](/molmil-images/mine/5b47) | 2-Oxoacid:Ferredoxin Oxidoreductase 2 from Sulfolobus tokodai - pyruvate complex | Descriptor: | 2-oxoacid--ferredoxin oxidoreductase alpha subunit, 2-oxoacid--ferredoxin oxidoreductase beta subunit, IRON/SULFUR CLUSTER, ... | Authors: | Yan, Z, Maruyama, A, Arakawa, T, Fushinobu, S, Wakagi, T. | Deposit date: | 2016-04-01 | Release date: | 2016-09-28 | Last modified: | 2023-11-15 | Method: | X-RAY DIFFRACTION (2.2 Å) | Cite: | Crystal structures of archaeal 2-oxoacid:ferredoxin oxidoreductases from Sulfolobus tokodaii Sci Rep, 6, 2016
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7YQS
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![BU of 7yqs by Molmil](/molmil-images/mine/7yqs) | Neutron structure of a L-rhamnose-alpha-1,4-D-glucuronate lyase from Fusarium oxysporum 12S, L-Rha complex | Descriptor: | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ACETATE ION, CALCIUM ION, ... | Authors: | Yano, N, Kondo, T, Kusaka, K, Yamada, T, Arakawa, T, Sakamoto, T, Fushinobu, S. | Deposit date: | 2022-08-08 | Release date: | 2023-08-09 | Last modified: | 2024-03-27 | Method: | NEUTRON DIFFRACTION (1.25 Å), X-RAY DIFFRACTION | Cite: | Charge neutralization and beta-elimination cleavage mechanism of family 42 L-rhamnose-alpha-1,4-D-glucuronate lyase revealed using neutron crystallography. J.Biol.Chem., 300, 2024
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8I4D
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![BU of 8i4d by Molmil](/molmil-images/mine/8i4d) | X-ray structure of a L-rhamnose-alpha-1,4-D-glucuronate lyase from Fusarium oxysporum 12S, L-Rha complex at 100K | Descriptor: | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ACETATE ION, CALCIUM ION, ... | Authors: | Yano, N, Kondo, T, Kusaka, K, Yamada, T, Arakawa, T, Sakamoto, T, Fushinobu, S. | Deposit date: | 2023-01-19 | Release date: | 2024-01-24 | Last modified: | 2024-03-27 | Method: | X-RAY DIFFRACTION (1.06 Å) | Cite: | Charge neutralization and beta-elimination cleavage mechanism of family 42 L-rhamnose-alpha-1,4-D-glucuronate lyase revealed using neutron crystallography. J.Biol.Chem., 300, 2024
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8IBR
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![BU of 8ibr by Molmil](/molmil-images/mine/8ibr) | Crystal structure of GH42 beta-galactosidase BiBga42A from Bifidobacterium longum subspecies infantis in complex with glycerol | Descriptor: | Beta-galactosidase, DI(HYDROXYETHYL)ETHER, GLYCEROL | Authors: | Hidaka, M, Fushinobu, S, Gotoh, A, Katayama, T. | Deposit date: | 2023-02-10 | Release date: | 2023-06-07 | Last modified: | 2023-12-20 | Method: | X-RAY DIFFRACTION (1.7 Å) | Cite: | Substrate recognition mode of a glycoside hydrolase family 42 beta-galactosidase from Bifidobacterium longum subspecies infantis ( Bi Bga42A) revealed by crystallographic and mutational analyses. Microbiome Res Rep, 2, 2023
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8IBT
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![BU of 8ibt by Molmil](/molmil-images/mine/8ibt) | Crystal structure of GH42 beta-galactosidase BiBga42A from Bifidobacterium longum subspecies infantis E318S mutant in complex with lacto-N-tetraose | Descriptor: | Beta-galactosidase, beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-beta-D-galactopyranose-(1-4)-beta-D-glucopyranose | Authors: | Hidaka, M, Fushinobu, S, Gotoh, A, Katayama, T. | Deposit date: | 2023-02-10 | Release date: | 2023-06-07 | Last modified: | 2023-12-20 | Method: | X-RAY DIFFRACTION (2.2 Å) | Cite: | Substrate recognition mode of a glycoside hydrolase family 42 beta-galactosidase from Bifidobacterium longum subspecies infantis ( Bi Bga42A) revealed by crystallographic and mutational analyses. Microbiome Res Rep, 2, 2023
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8IBS
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![BU of 8ibs by Molmil](/molmil-images/mine/8ibs) | Crystal structure of GH42 beta-galactosidase BiBga42A from Bifidobacterium longum subspecies infantis E160A/E318A mutant in complex with galactose | Descriptor: | Beta-galactosidase, alpha-D-galactopyranose | Authors: | Hidaka, M, Fushinobu, S, Gotoh, A, Katayama, T. | Deposit date: | 2023-02-10 | Release date: | 2023-06-07 | Last modified: | 2023-12-20 | Method: | X-RAY DIFFRACTION (1.9 Å) | Cite: | Substrate recognition mode of a glycoside hydrolase family 42 beta-galactosidase from Bifidobacterium longum subspecies infantis ( Bi Bga42A) revealed by crystallographic and mutational analyses. Microbiome Res Rep, 2, 2023
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5B0P
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![BU of 5b0p by Molmil](/molmil-images/mine/5b0p) | Beta-1,2-Mannobiose phosphorylase from Listeria innocua - glycerol complex | Descriptor: | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, GLYCEROL, Lin0857 protein, ... | Authors: | Tsuda, T, Arakawa, T, Fushinobu, S. | Deposit date: | 2015-11-02 | Release date: | 2015-12-02 | Last modified: | 2023-11-08 | Method: | X-RAY DIFFRACTION (1.9 Å) | Cite: | Characterization and crystal structure determination of beta-1,2-mannobiose phosphorylase from Listeria innocua Febs Lett., 589, 2015
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5AYI
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![BU of 5ayi by Molmil](/molmil-images/mine/5ayi) | Crystal structure of GH1 Beta-glucosidase TD2F2 N223Q mutant | Descriptor: | 2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID, BETA-GLUCOSIDASE, GLYCEROL, ... | Authors: | Jo, T, Manninen, J.A, Matsuzawa, T, Uchiyama, T, Yaoi, K, Arakawa, T, Fushinobu, S. | Deposit date: | 2015-08-21 | Release date: | 2016-04-27 | Last modified: | 2023-11-08 | Method: | X-RAY DIFFRACTION (1.85 Å) | Cite: | Crystal structure and identification of a key amino acid for glucose tolerance, substrate specificity, and transglycosylation activity of metagenomic beta-glucosidase Td2F2 Febs J., 283, 2016
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5B0S
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![BU of 5b0s by Molmil](/molmil-images/mine/5b0s) | Beta-1,2-Mannobiose phosphorylase from Listeria innocua - beta-1,2-mannotriose complex | Descriptor: | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, GLYCEROL, Lin0857 protein, ... | Authors: | Tsuda, T, Arakawa, T, Fushinobu, S. | Deposit date: | 2015-11-02 | Release date: | 2015-12-02 | Last modified: | 2023-11-08 | Method: | X-RAY DIFFRACTION (2.1 Å) | Cite: | Characterization and crystal structure determination of beta-1,2-mannobiose phosphorylase from Listeria innocua Febs Lett., 589, 2015
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5AYB
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![BU of 5ayb by Molmil](/molmil-images/mine/5ayb) | Crystal structure of GH1 Beta-Glucosidase TD2F2 N223G mutant | Descriptor: | 1,2-ETHANEDIOL, 2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID, BETA-GLUCOSIDASE, ... | Authors: | Jo, T, Manninen, J.A, Matsuzawa, T, Uchiyama, T, Yaoi, K, Arakawa, T, Fushinobu, S. | Deposit date: | 2015-08-12 | Release date: | 2016-04-27 | Last modified: | 2023-11-08 | Method: | X-RAY DIFFRACTION (1.8 Å) | Cite: | Crystal structure and identification of a key amino acid for glucose tolerance, substrate specificity, and transglycosylation activity of metagenomic beta-glucosidase Td2F2 Febs J., 283, 2016
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1UGQ
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![BU of 1ugq by Molmil](/molmil-images/mine/1ugq) | Crystal structure of apoenzyme of Co-type nitrile hydratase | Descriptor: | Nitrile Hydratase alpha subunit, Nitrile Hydratase beta subunit | Authors: | Miyanaga, A, Fushinobu, S, Ito, K, Shoun, H, Wakagi, T. | Deposit date: | 2003-06-17 | Release date: | 2004-06-17 | Last modified: | 2023-12-27 | Method: | X-RAY DIFFRACTION (2 Å) | Cite: | Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding Eur.J.Biochem., 271, 2004
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1UGR
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![BU of 1ugr by Molmil](/molmil-images/mine/1ugr) | Crystal structure of aT109S mutant of Co-type nitrile hydratase | Descriptor: | COBALT (II) ION, Nitrile Hydratase alpha subunit, Nitrile Hydratase beta subunit | Authors: | Miyanaga, A, Fushinobu, S, Ito, K, Shoun, H, Wakagi, T. | Deposit date: | 2003-06-17 | Release date: | 2004-06-17 | Last modified: | 2023-12-27 | Method: | X-RAY DIFFRACTION (1.8 Å) | Cite: | Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding Eur.J.Biochem., 271, 2004
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7DFS
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![BU of 7dfs by Molmil](/molmil-images/mine/7dfs) | Crystal structure of a novel 4-O-alpha-L-rhamnosyl-beta-D-glucuronidase from Fusarium oxysporum 12S - Rha-GlcA complex | Descriptor: | 2-acetamido-2-deoxy-beta-D-glucopyranose, 4-O-alpha-L-rhamnosyl-beta-D-glucuronidase, alpha-D-mannopyranose, ... | Authors: | Kondo, T, Arakawa, T, Fushinobu, S, Sakamoto, T. | Deposit date: | 2020-11-09 | Release date: | 2021-03-17 | Last modified: | 2024-04-03 | Method: | X-RAY DIFFRACTION (1.49 Å) | Cite: | Biochemical and structural characterization of a novel 4-O-alpha-l-rhamnosyl-beta-d-glucuronidase from Fusarium oxysporum. Febs J., 288, 2021
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7DFQ
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![BU of 7dfq by Molmil](/molmil-images/mine/7dfq) | Crystal Structure of a novel 4-O-alpha-L-rhamnosyl-beta-D-glucuronidase from Fusarium oxysporum 12S, ligand-free form | Descriptor: | 2-acetamido-2-deoxy-beta-D-glucopyranose, 4-O-alpha-L-rhamnosyl-beta-D-glucuronidase | Authors: | Kondo, T, Arakawa, T, Fushinobu, S, Sakamoto, T. | Deposit date: | 2020-11-09 | Release date: | 2021-03-17 | Last modified: | 2021-08-25 | Method: | X-RAY DIFFRACTION (1.51 Å) | Cite: | Biochemical and structural characterization of a novel 4-O-alpha-l-rhamnosyl-beta-d-glucuronidase from Fusarium oxysporum. Febs J., 288, 2021
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5B0R
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![BU of 5b0r by Molmil](/molmil-images/mine/5b0r) | Beta-1,2-Mannobiose phosphorylase from Listeria innocua - beta-1,2-mannobiose complex | Descriptor: | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, GLYCEROL, Lin0857 protein, ... | Authors: | Tsuda, T, Arakawa, T, Fushinobu, S. | Deposit date: | 2015-11-02 | Release date: | 2015-12-02 | Last modified: | 2023-11-08 | Method: | X-RAY DIFFRACTION (1.8 Å) | Cite: | Characterization and crystal structure determination of beta-1,2-mannobiose phosphorylase from Listeria innocua Febs Lett., 589, 2015
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5B0Q
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![BU of 5b0q by Molmil](/molmil-images/mine/5b0q) | beta-1,2-Mannobiose phosphorylase from Listeria innocua - mannose complex | Descriptor: | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, Lin0857 protein, SULFATE ION, ... | Authors: | Tsuda, T, Arakawa, T, Fushinobu, S. | Deposit date: | 2015-11-02 | Release date: | 2015-12-02 | Last modified: | 2023-11-08 | Method: | X-RAY DIFFRACTION (2.3 Å) | Cite: | Characterization and crystal structure determination of beta-1,2-mannobiose phosphorylase from Listeria innocua Febs Lett., 589, 2015
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6LCE
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![BU of 6lce by Molmil](/molmil-images/mine/6lce) | |