8IBM
| Sulfate bound form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H and S176A inactivation | 分子名称: | Alpha/beta hydrolase family protein, CALCIUM ION, SULFATE ION | 著者 | Emori, M, Numoto, N, Kamiya, N, Oda, M. | 登録日 | 2023-02-10 | 公開日 | 2023-03-15 | 実験手法 | X-RAY DIFFRACTION (2.2 Å) | 主引用文献 | Improvement of thermostability and activity of PET-degrading enzyme Cut190 towards a detailed understanding and application of the enzymatic reaction mechanism. Biorxiv, 2023
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8IBL
| MES bound form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H and S176A inactivation | 分子名称: | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, Alpha/beta hydrolase family protein, CALCIUM ION, ... | 著者 | Emori, M, Numoto, N, Kamiya, N, Oda, M. | 登録日 | 2023-02-10 | 公開日 | 2023-03-15 | 実験手法 | X-RAY DIFFRACTION (2.6 Å) | 主引用文献 | Improvement of thermostability and activity of PET-degrading enzyme Cut190 towards a detailed understanding and application of the enzymatic reaction mechanism. Biorxiv, 2023
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7CTS
| Open form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H and S176A inactivation | 分子名称: | 1,4-DIETHYLENE DIOXIDE, Alpha/beta hydrolase family protein, BICINE, ... | 著者 | Emori, M, Numoto, N, Senga, A, Bekker, G.J, Kamiya, N, Ito, N, Kawai, F, Oda, M. | 登録日 | 2020-08-20 | 公開日 | 2021-02-03 | 最終更新日 | 2023-11-29 | 実験手法 | X-RAY DIFFRACTION (1.1 Å) | 主引用文献 | Structural basis of mutants of PET-degrading enzyme from Saccharomonospora viridis AHK190 with high activity and thermal stability. Proteins, 89, 2021
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7CTR
| Closed form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H | 分子名称: | 1,4-DIETHYLENE DIOXIDE, Alpha/beta hydrolase family protein | 著者 | Emori, M, Numoto, N, Senga, A, Bekker, G.J, Kamiya, N, Ito, N, Kawai, F, Oda, M. | 登録日 | 2020-08-20 | 公開日 | 2021-02-03 | 最終更新日 | 2023-11-29 | 実験手法 | X-RAY DIFFRACTION (1.2 Å) | 主引用文献 | Structural basis of mutants of PET-degrading enzyme from Saccharomonospora viridis AHK190 with high activity and thermal stability. Proteins, 89, 2021
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