8EA4
 
 | | V-K CAST Transpososome from Scytonema hofmanni, minor configuration | | Descriptor: | 30S ribosomal protein S15, ADENOSINE-5'-TRIPHOSPHATE, Cas12k, ... | | Authors: | Rizo, A.R, Park, J.-U, Tsai, A.W, Kellogg, E.K. | | Deposit date: | 2022-08-27 | | Release date: | 2022-11-30 | | Last modified: | 2024-06-19 | | Method: | ELECTRON MICROSCOPY (3 Å) | | Cite: | Structures of the holo CRISPR RNA-guided transposon integration complex.
Nature, 613, 2023
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8EA3
 | | V-K CAST Transpososome from Scytonema hofmanni, major configuration | | Descriptor: | 30S ribosomal protein S15, ADENOSINE-5'-TRIPHOSPHATE, Cas12k, ... | | Authors: | Rizo, A.N, Park, J.-U, Tsai, A.W, Kellogg, E.H. | | Deposit date: | 2022-08-27 | | Release date: | 2022-11-30 | | Last modified: | 2024-06-19 | | Method: | ELECTRON MICROSCOPY (3.7 Å) | | Cite: | Structures of the holo CRISPR RNA-guided transposon integration complex.
Nature, 613, 2023
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7SVW
 | | Strand-transfer complex of TnsB from ShCAST | | Descriptor: | MAGNESIUM ION, STC_LE_For, STC_LE_Rev1, ... | | Authors: | Park, J, Tsai, A.W.T, Kellogg, E.H. | | Deposit date: | 2021-11-19 | | Release date: | 2022-08-10 | | Last modified: | 2024-02-28 | | Method: | ELECTRON MICROSCOPY (3.69 Å) | | Cite: | Mechanistic details of CRISPR-associated transposon recruitment and integration revealed by cryo-EM.
Proc.Natl.Acad.Sci.USA, 119, 2022
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7SVV
 | | TnsBctd-TnsC complex | | Descriptor: | DNA (5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3'), DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), MAGNESIUM ION, ... | | Authors: | Park, J, Tsai, A.W.T, Kellogg, E.H. | | Deposit date: | 2021-11-19 | | Release date: | 2022-08-10 | | Last modified: | 2024-02-28 | | Method: | ELECTRON MICROSCOPY (3.54 Å) | | Cite: | Mechanistic details of CRISPR-associated transposon recruitment and integration revealed by cryo-EM.
Proc.Natl.Acad.Sci.USA, 119, 2022
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7M99
 | | ATPgS bound TnsC filament from ShCAST system | | Descriptor: | DNA (5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3'), DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), MAGNESIUM ION, ... | | Authors: | Park, J, Tsai, A.W.L, Mehrotra, E, Kellogg, E.H. | | Deposit date: | 2021-03-30 | | Release date: | 2021-07-28 | | Last modified: | 2024-05-29 | | Method: | ELECTRON MICROSCOPY (3.2 Å) | | Cite: | Structural basis for target site selection in RNA-guided DNA transposition systems.
Science, 373, 2021
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7M9A
 | | ADP-AlF3 bound TnsC structure from ShCAST system | | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, DNA (27-MER), TnsC | | Authors: | Park, J, Tsai, A.W.L, Mehrotra, E, Kellogg, E.H. | | Deposit date: | 2021-03-30 | | Release date: | 2021-07-28 | | Last modified: | 2024-05-29 | | Method: | ELECTRON MICROSCOPY (3.9 Å) | | Cite: | Structural basis for target site selection in RNA-guided DNA transposition systems.
Science, 373, 2021
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7M9C
 | | ADP-AlF3 bound TnsC structure in open form | | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, DNA (34-MER), TnsC | | Authors: | Park, J, Tsai, A.W.L, Mehrotra, E, Kellogg, E.H. | | Deposit date: | 2021-03-30 | | Release date: | 2021-07-28 | | Last modified: | 2024-05-29 | | Method: | ELECTRON MICROSCOPY (4.2 Å) | | Cite: | Structural basis for target site selection in RNA-guided DNA transposition systems.
Science, 373, 2021
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7M9B
 | | ADP-AlF3 bound TnsC structure in closed form | | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, DNA (27-MER), TnsC | | Authors: | Park, J, Tsai, A.W.L, Mehrotra, E, Kellogg, E.H. | | Deposit date: | 2021-03-30 | | Release date: | 2021-07-28 | | Last modified: | 2024-05-29 | | Method: | ELECTRON MICROSCOPY (3.8 Å) | | Cite: | Structural basis for target site selection in RNA-guided DNA transposition systems.
Science, 373, 2021
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7N6I
 | | ATP-bound TnsC-TniQ complex from ShCAST system | | Descriptor: | ADENOSINE-5'-TRIPHOSPHATE, DNA (5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3'), DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), ... | | Authors: | Park, J, Tsai, A.W.L, Mehrotra, E, Kellogg, E.H. | | Deposit date: | 2021-06-08 | | Release date: | 2021-07-28 | | Last modified: | 2024-05-29 | | Method: | ELECTRON MICROSCOPY (3.9 Å) | | Cite: | Structural basis for target site selection in RNA-guided DNA transposition systems.
Science, 373, 2021
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7SVU
 | | TnsBctd-TnsC-TniQ complex | | Descriptor: | ADENOSINE-5'-TRIPHOSPHATE, DNA (28-MER), DNA (29-MER), ... | | Authors: | Park, J, Tsai, A.W.T, Kellogg, E.H. | | Deposit date: | 2021-11-19 | | Release date: | 2022-11-23 | | Last modified: | 2024-06-05 | | Method: | ELECTRON MICROSCOPY (3.5 Å) | | Cite: | Structures of the holo CRISPR RNA-guided transposon integration complex
Nature, 613, 2023
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