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7AM4
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BU of 7am4 by Molmil
Crystal structure of Peptiligase mutant - L217H/M222P
Descriptor: GLYCEROL, SULFATE ION, Subtilisin BPN'
Authors:Rozeboom, H.J, Janssen, D.J.
Deposit date:2020-10-08
Release date:2021-02-17
Last modified:2024-05-01
Method:X-RAY DIFFRACTION (1.81 Å)
Cite:From thiol-subtilisin to omniligase: Design and structure of a broadly applicable peptide ligase.
Comput Struct Biotechnol J, 19, 2021
7AM5
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BU of 7am5 by Molmil
Crystal structure of Peptiligase mutant - L217H/M222P/A225N
Descriptor: SODIUM ION, Subtilisin BPN'
Authors:Rozeboom, H.J, Janssen, D.J.
Deposit date:2020-10-08
Release date:2021-02-17
Last modified:2021-03-24
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:From thiol-subtilisin to omniligase: Design and structure of a broadly applicable peptide ligase.
Comput Struct Biotechnol J, 19, 2021
7AM7
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BU of 7am7 by Molmil
Crystal structure of Peptiligase mutant - M222P/L217H/A225N/F189W/N218D
Descriptor: Eglin C fragment, GLYCEROL, SULFATE ION, ...
Authors:Rozeboom, H.J, Janssen, D.J.
Deposit date:2020-10-08
Release date:2021-02-17
Last modified:2024-05-01
Method:X-RAY DIFFRACTION (2.61 Å)
Cite:From thiol-subtilisin to omniligase: Design and structure of a broadly applicable peptide ligase.
Comput Struct Biotechnol J, 19, 2021
7OG2
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BU of 7og2 by Molmil
Crystal structure of Pseudoalteromonas luteoviolacea L-amino acid oxidase
Descriptor: Amine oxidoreductase, CHLORIDE ION, FLAVIN-ADENINE DINUCLEOTIDE, ...
Authors:Rozeboom, H.J, Savino, S, Fraaije, M.W.
Deposit date:2021-05-06
Release date:2021-11-10
Last modified:2024-01-31
Method:X-RAY DIFFRACTION (2.8 Å)
Cite:Kinetic and Structural Properties of a Robust Bacterial L-Amino Acid Oxidase
Catalysts, 2021
7PUO
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BU of 7puo by Molmil
Structure of a fused 4-OT variant engineered for asymmetric Michael addition reactions
Descriptor: 2-hydroxymuconate tautomerase,Chains: A,B,C,D,E,F,2-hydroxymuconate tautomerase, CHLORIDE ION, GLYCEROL
Authors:Rozeboom, H.J, Thunnissen, A.M.W.H, Poelarends, G.J.
Deposit date:2021-09-30
Release date:2022-01-26
Last modified:2024-01-31
Method:X-RAY DIFFRACTION (2.35 Å)
Cite:Gene Fusion and Directed Evolution to Break Structural Symmetry and Boost Catalysis by an Oligomeric C-C Bond-Forming Enzyme.
Angew.Chem.Int.Ed.Engl., 61, 2022
8APZ
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BU of 8apz by Molmil
Crystal structure of wild-type L-N-Carbamoylase from Sinorhizobium meliloti
Descriptor: ACETATE ION, D-ORNITHINE, FE (III) ION, ...
Authors:Rozeboom, H.J, Mayer, C.
Deposit date:2022-08-11
Release date:2022-11-16
Last modified:2024-05-01
Method:X-RAY DIFFRACTION (1.75 Å)
Cite:Selecting Better Biocatalysts by Complementing Recoded Bacteria.
Angew.Chem.Int.Ed.Engl., 62, 2023
8AQ0
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BU of 8aq0 by Molmil
Crystal structure of L-N-Carbamoylase from Sinorhizobium meliloti mutant L217G/F329C
Descriptor: (2~{S})-2-(aminocarbonylamino)-3-(4-hydroxyphenyl)propanoic acid, CHLORIDE ION, FE (III) ION, ...
Authors:Rozeboom, H.J, Mayer, C.
Deposit date:2022-08-11
Release date:2022-11-16
Last modified:2024-05-01
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Selecting Better Biocatalysts by Complementing Recoded Bacteria.
Angew.Chem.Int.Ed.Engl., 62, 2023
5OX2
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BU of 5ox2 by Molmil
Crystal structure of thymoligase, a substrate-tailored peptiligase variant
Descriptor: Fragment of prodomain, SULFATE ION, Subtilisin BPN'
Authors:Rozeboom, H.J, Janssen, D.B.
Deposit date:2017-09-05
Release date:2018-01-10
Last modified:2018-04-18
Method:X-RAY DIFFRACTION (2.24 Å)
Cite:Design of a substrate-tailored peptiligase variant for the efficient synthesis of thymosin-alpha1.
Org. Biomol. Chem., 16, 2018
6G4B
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BU of 6g4b by Molmil
Crystal structure of the omega transaminase from Pseudomonas jessenii in the apo form, crystallized from succinate
Descriptor: Aspartate aminotransferase family protein, GLYCEROL, SUCCINIC ACID
Authors:Rozeboom, H.J, Janssen, D.B.
Deposit date:2018-03-27
Release date:2019-04-10
Last modified:2024-01-17
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Biochemical properties of a Pseudomonas aminotransferase involved in caprolactam metabolism.
Febs J., 286, 2019
6G4D
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BU of 6g4d by Molmil
Crystal structure of the omega TRANSAMINASE FROM PSEUDOMONAS Jessenii in complex with PLP
Descriptor: Aspartate aminotransferase family protein, GLYCEROL, PYRIDOXAL-5'-PHOSPHATE
Authors:Rozeboom, H.J, Janssen, D.B.
Deposit date:2018-03-27
Release date:2019-04-10
Last modified:2024-05-01
Method:X-RAY DIFFRACTION (2.15 Å)
Cite:Biochemical properties of a Pseudomonas aminotransferase involved in caprolactam metabolism.
Febs J., 286, 2019
6G4E
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BU of 6g4e by Molmil
Crystal structure of the omega TRANSAMINASE FROM PSEUDOMONAS Jessenii in complex with PLP and 6-aminohexanoate (6-ACA)
Descriptor: 6-AMINOHEXANOIC ACID, Aspartate aminotransferase family protein, GLYCEROL, ...
Authors:Rozeboom, H.J, Janssen, D.B.
Deposit date:2018-03-27
Release date:2019-04-10
Last modified:2024-05-01
Method:X-RAY DIFFRACTION (2.45 Å)
Cite:Biochemical properties of a Pseudomonas aminotransferase involved in caprolactam metabolism.
Febs J., 286, 2019
6I9G
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BU of 6i9g by Molmil
Crystal structure of encapsulin from Mycolicibacterium hassiacum
Descriptor: GLYCEROL, Linocin-M18, SULFATE ION
Authors:Rozeboom, H.J, Fraaije, M.W.
Deposit date:2018-11-23
Release date:2019-12-04
Last modified:2024-01-24
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Structure of a robust bacterial protein cage and its application as a versatile biocatalytic platform through enzyme encapsulation.
Biochem.Biophys.Res.Commun., 529, 2020
3BMV
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BU of 3bmv by Molmil
Cyclodextrin glycosyl transferase from Thermoanerobacterium thermosulfurigenes EM1 mutant S77P
Descriptor: CALCIUM ION, Cyclomaltodextrin glucanotransferase, GLYCEROL, ...
Authors:Rozeboom, H.J, van Oosterwijk, N, Dijkstra, B.W.
Deposit date:2007-12-13
Release date:2008-05-27
Last modified:2023-11-01
Method:X-RAY DIFFRACTION (1.6 Å)
Cite:Elimination of competing hydrolysis and coupling side reactions of a cyclodextrin glucanotransferase by directed evolution.
Biochem.J., 413, 2008
3BMW
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BU of 3bmw by Molmil
Cyclodextrin glycosyl transferase from Thermoanerobacterium thermosulfurigenes EM1 mutant S77P complexed with a maltoheptaose inhibitor
Descriptor: 6-AMINO-4-HYDROXYMETHYL-CYCLOHEX-4-ENE-1,2,3-TRIOL, CALCIUM ION, CHLORIDE ION, ...
Authors:Rozeboom, H.J, van Oosterwijk, N, Dijkstra, B.W.
Deposit date:2007-12-13
Release date:2008-05-27
Last modified:2023-11-29
Method:X-RAY DIFFRACTION (1.6 Å)
Cite:Elimination of competing hydrolysis and coupling side reactions of a cyclodextrin glucanotransferase by directed evolution.
Biochem.J., 413, 2008
5K8E
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BU of 5k8e by Molmil
Xylooligosaccharide oxidase from Myceliophthora thermophila C1
Descriptor: 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, 2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ...
Authors:Rozeboom, H.J, Ferrari, A.R, Fraaije, M.W.
Deposit date:2016-05-30
Release date:2016-09-21
Last modified:2024-01-10
Method:X-RAY DIFFRACTION (1.93 Å)
Cite:Discovery of a Xylooligosaccharide Oxidase from Myceliophthora thermophila C1.
J.Biol.Chem., 291, 2016
5L6F
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BU of 5l6f by Molmil
Xylooligosaccharide oxidase from Myceliophthora thermophila C1 in complex with Xylobiose
Descriptor: 2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, DI(HYDROXYETHYL)ETHER, ...
Authors:Rozeboom, H.J, Ferrari, A.R, Fraaije, M.W.
Deposit date:2016-05-30
Release date:2016-09-21
Last modified:2024-01-10
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Discovery of a Xylooligosaccharide Oxidase from Myceliophthora thermophila C1.
J.Biol.Chem., 291, 2016
5L6G
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BU of 5l6g by Molmil
Xylooligosaccharide oxidase from Myceliophthora thermophila C1 in complex with Xylose
Descriptor: 2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, FAD linked oxidase-like protein, ...
Authors:Rozeboom, H.J, Ferrari, A.R, Fraaije, M.W.
Deposit date:2016-05-30
Release date:2016-09-21
Last modified:2024-01-10
Method:X-RAY DIFFRACTION (1.79 Å)
Cite:Discovery of a Xylooligosaccharide Oxidase from Myceliophthora thermophila C1.
J.Biol.Chem., 291, 2016
5NH6
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BU of 5nh6 by Molmil
Crystal structure of xylose isomerase from Piromyces E2 Complexed with one Mg2+ ion and xylitol
Descriptor: MAGNESIUM ION, SULFATE ION, Xylitol, ...
Authors:Rozeboom, H.J, Janssen, D.B.
Deposit date:2017-03-21
Release date:2017-11-01
Last modified:2024-05-01
Method:X-RAY DIFFRACTION (1.75 Å)
Cite:Metal Dependence of the Xylose Isomerase from Piromyces sp. E2 Explored by Activity Profiling and Protein Crystallography.
Biochemistry, 56, 2017
6TP2
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BU of 6tp2 by Molmil
Crystal structure of Bacillus paralicheniformis alpha-amylase in complex with beta-cyclodextrin
Descriptor: Amylase, CALCIUM ION, Cycloheptakis-(1-4)-(alpha-D-glucopyranose), ...
Authors:Rozeboom, H.J, Janssen, D.B.
Deposit date:2019-12-12
Release date:2020-10-14
Last modified:2024-01-24
Method:X-RAY DIFFRACTION (1.94 Å)
Cite:Characterization of the starch surface binding site on Bacillus paralicheniformis alpha-amylase.
Int.J.Biol.Macromol., 165, 2020
6T8F
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BU of 6t8f by Molmil
Crystal structure of mutant xylose isomerase (V270A/A273G) from Piromyces E2 grown in yeast, in complex with xylose
Descriptor: CALCIUM ION, D-xylose, SULFATE ION, ...
Authors:Rozeboom, H.J, Janssen, D.B.
Deposit date:2019-10-24
Release date:2020-01-29
Last modified:2024-01-24
Method:X-RAY DIFFRACTION (2 Å)
Cite:Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance.
Biotechnol Biofuels, 13, 2020
6T8E
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BU of 6t8e by Molmil
Crystal structure of native xylose isomerase from Piromyces E2 grown in yeast, in complex with xylose
Descriptor: CALCIUM ION, D-xylose, SULFATE ION, ...
Authors:Rozeboom, H.J, Janssen, D.B.
Deposit date:2019-10-24
Release date:2020-01-29
Last modified:2024-01-24
Method:X-RAY DIFFRACTION (1.86 Å)
Cite:Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance.
Biotechnol Biofuels, 13, 2020
6TOZ
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BU of 6toz by Molmil
Crystal structure of Bacillus paralicheniformis alpha-amylase in complex with acarbose
Descriptor: 4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ACETIC ACID, Amylase, ...
Authors:Rozeboom, H.J, Janssen, D.B.
Deposit date:2019-12-12
Release date:2020-10-14
Last modified:2024-01-24
Method:X-RAY DIFFRACTION (1.94 Å)
Cite:Characterization of the starch surface binding site on Bacillus paralicheniformis alpha-amylase.
Int.J.Biol.Macromol., 165, 2020
6TP0
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BU of 6tp0 by Molmil
Crystal structure of Bacillus paralicheniformis alpha-amylase in complex with maltose
Descriptor: ACETIC ACID, Amylase, CALCIUM ION, ...
Authors:Rozeboom, H.J, Janssen, D.B.
Deposit date:2019-12-12
Release date:2020-10-14
Last modified:2024-01-24
Method:X-RAY DIFFRACTION (2.04 Å)
Cite:Characterization of the starch surface binding site on Bacillus paralicheniformis alpha-amylase.
Int.J.Biol.Macromol., 165, 2020
6TOY
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BU of 6toy by Molmil
Crystal structure of Bacillus paralicheniformis wild-type alpha-amylase
Descriptor: ACETIC ACID, Amylase, CALCIUM ION, ...
Authors:Rozeboom, H.J, Janssen, D.B.
Deposit date:2019-12-12
Release date:2020-10-14
Last modified:2024-01-24
Method:X-RAY DIFFRACTION (1.95 Å)
Cite:Characterization of the starch surface binding site on Bacillus paralicheniformis alpha-amylase.
Int.J.Biol.Macromol., 165, 2020
6TP1
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BU of 6tp1 by Molmil
Crystal structure of Bacillus paralicheniformis alpha-amylase in complex with maltotetraose
Descriptor: ACETIC ACID, Amylase, CALCIUM ION, ...
Authors:Rozeboom, H.J, Janssen, D.B.
Deposit date:2019-12-12
Release date:2020-10-14
Last modified:2024-01-24
Method:X-RAY DIFFRACTION (1.94 Å)
Cite:Characterization of the starch surface binding site on Bacillus paralicheniformis alpha-amylase.
Int.J.Biol.Macromol., 165, 2020

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