3NRX
 
 | | Insights into anti-parallel microtubule crosslinking by PRC1, a conserved non-motor microtubule binding protein | | Descriptor: | Protein regulator of cytokinesis 1 | | Authors: | Kapoor, T.M, Subramanian, R, Wilson-Kubalek, E.M, Arthur, C.P, Bick, M.J, Campbell, E.A, Darst, S.A, Milligan, R.A. | | Deposit date: | 2010-06-30 | | Release date: | 2010-08-18 | | Last modified: | 2023-09-06 | | Method: | X-RAY DIFFRACTION (1.75 Å) | | Cite: | Insights into Antiparallel Microtubule Crosslinking by PRC1, a Conserved Nonmotor Microtubule Binding Protein.
Cell(Cambridge,Mass.), 142, 2010
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3NRY
 | | Insights into anti-parallel microtubule crosslinking by PRC1, a conserved microtubule binding protein | | Descriptor: | Protein regulator of cytokinesis 1 | | Authors: | Kapoor, T.M, Subramanian, R, Wilson-Kubalek, E.M, Arthur, C.P, Bick, M.J, Campbell, E.A, Darst, S.A, Milligan, R.A. | | Deposit date: | 2010-07-01 | | Release date: | 2010-08-25 | | Last modified: | 2023-12-27 | | Method: | X-RAY DIFFRACTION (2 Å) | | Cite: | Insights into Antiparallel Microtubule Crosslinking by PRC1, a Conserved Nonmotor Microtubule Binding Protein.
Cell(Cambridge,Mass.), 142, 2010
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3NIB
 | | Teg14 Apo | | Descriptor: | 2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID, GLYCEROL, Teg14 | | Authors: | Bick, M.J, Banik, J.J, Darst, S.A, Brady, S.F. | | Deposit date: | 2010-06-15 | | Release date: | 2010-12-08 | | Last modified: | 2023-09-06 | | Method: | X-RAY DIFFRACTION (2.7 Å) | | Cite: | The 2.7 A resolution structure of the glycopeptide sulfotransferase Teg14
Acta Crystallogr.,Sect.D, 66, 2010
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1OR7
 | | Crystal Structure of Escherichia coli sigmaE with the Cytoplasmic Domain of its Anti-sigma RseA | | Descriptor: | RNA polymerase sigma-E factor, Sigma-E factor negative regulatory protein | | Authors: | Campbell, E.A, Tupy, J.L, Gruber, T.M, Wang, S, Sharp, M.M, Gross, C.A, Darst, S.A. | | Deposit date: | 2003-03-12 | | Release date: | 2003-04-15 | | Last modified: | 2024-02-14 | | Method: | X-RAY DIFFRACTION (2 Å) | | Cite: | Crystal structure of Escherichia coli sigmaE with the cytoplasmic domain of its anti-sigma RseA.
Mol.Cell, 11, 2003
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1RIO
 | | Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA | | Descriptor: | (4S)-2-METHYL-2,4-PENTANEDIOL, 27-MER, CALCIUM ION, ... | | Authors: | Jain, D, Nickels, B.E, Sun, L, Hochschild, A, Darst, S.A. | | Deposit date: | 2003-11-17 | | Release date: | 2004-01-27 | | Last modified: | 2011-07-13 | | Method: | X-RAY DIFFRACTION (2.3 Å) | | Cite: | Structure of a ternary transcription activation complex.
Mol.Cell, 13, 2004
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1RP3
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1SC5
 | | Sigma-28(FliA)/FlgM complex | | Descriptor: | RNA polymerase sigma factor FliA, anti-sigma factor FlgM | | Authors: | Sorenson, M.K, Ray, S.S, Darst, S.A. | | Deposit date: | 2004-02-11 | | Release date: | 2004-04-06 | | Last modified: | 2024-02-14 | | Method: | X-RAY DIFFRACTION (3.26 Å) | | Cite: | Crystal structure of the flagellar sigma/anti-sigma complex sigma(28)/FlgM reveals an intact sigma factor in an inactive conformation.
Mol.Cell, 14, 2004
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4KI2
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6OUL
 | | Cryo-EM structure of Escherichia coli RNAP polymerase bound to rpsTP2 promoter DNA | | Descriptor: | CHAPSO, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | | Authors: | Chen, J, Chiu, C.E, Campbell, E.A, Darst, S.A. | | Deposit date: | 2019-05-04 | | Release date: | 2020-02-26 | | Last modified: | 2024-03-20 | | Method: | ELECTRON MICROSCOPY (3.4 Å) | | Cite: | E. coliTraR allosterically regulates transcription initiation by altering RNA polymerase conformation.
Elife, 8, 2019
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6P1K
 | | Cryo-EM structure of Escherichia coli sigma70 bound RNAP polymerase holoenzyme | | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | | Authors: | Chen, J, Chiu, C.E, Campbell, E.A, Darst, S.A. | | Deposit date: | 2019-05-20 | | Release date: | 2020-02-26 | | Last modified: | 2024-03-13 | | Method: | ELECTRON MICROSCOPY (4.05 Å) | | Cite: | E. coliTraR allosterically regulates transcription initiation by altering RNA polymerase conformation.
Elife, 8, 2019
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6OMF
 | | CryoEM structure of SigmaS-transcription initiation complex with activator Crl | | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | | Authors: | Jaramillo Cartagena, A, Darst, S.A, Campbell, E.A. | | Deposit date: | 2019-04-18 | | Release date: | 2019-08-28 | | Last modified: | 2024-03-13 | | Method: | ELECTRON MICROSCOPY (3.26 Å) | | Cite: | Structural basis for transcription activation by Crl through tethering of sigmaSand RNA polymerase.
Proc.Natl.Acad.Sci.USA, 116, 2019
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6PSU
 | | Escherichia coli RNA polymerase promoter unwinding intermediate (TRPi2) with TraR and rpsT P2 promoter | | Descriptor: | CHAPSO, DNA (85-MER), DNA-directed RNA polymerase subunit alpha, ... | | Authors: | Chen, J, Chiu, C.E, Campbell, E.A, Darst, S.A. | | Deposit date: | 2019-07-13 | | Release date: | 2020-03-25 | | Last modified: | 2024-03-20 | | Method: | ELECTRON MICROSCOPY (3.9 Å) | | Cite: | Stepwise Promoter Melting by Bacterial RNA Polymerase.
Mol.Cell, 78, 2020
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6PSR
 | | Escherichia coli RNA polymerase promoter unwinding intermediate (TRPi1) with TraR and rpsT P2 promoter | | Descriptor: | CHAPSO, DNA (85-MER), DNA-directed RNA polymerase subunit alpha, ... | | Authors: | Chen, J, Chiu, C.E, Campbell, E.A, Darst, S.A. | | Deposit date: | 2019-07-13 | | Release date: | 2020-03-25 | | Last modified: | 2024-03-20 | | Method: | ELECTRON MICROSCOPY (3.4 Å) | | Cite: | Stepwise Promoter Melting by Bacterial RNA Polymerase.
Mol.Cell, 78, 2020
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8EHI
 | | Cryo-EM structure of his-elemental paused elongation complex with an unfolded TL (2) | | Descriptor: | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... | | Authors: | Kang, J.Y, Chen, J, Llewellyn, E, Landick, R, Darst, S.A. | | Deposit date: | 2022-09-14 | | Release date: | 2023-03-01 | | Last modified: | 2024-06-19 | | Method: | ELECTRON MICROSCOPY (5.5 Å) | | Cite: | An ensemble of interconverting conformations of the elemental paused transcription complex creates regulatory options.
Proc.Natl.Acad.Sci.USA, 120, 2023
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8EHF
 | | Cryo-EM structure of his-elemental paused elongation complex with an unfolded TL (1) | | Descriptor: | (3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-10,13-dimethyl-17-[(2R)-pentan-2-yl]-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthrene-3,7,12-triol, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | | Authors: | Kang, J.Y, Chen, J, Llewellyn, E, Landick, R, Darst, S.A. | | Deposit date: | 2022-09-14 | | Release date: | 2023-03-01 | | Last modified: | 2024-06-19 | | Method: | ELECTRON MICROSCOPY (3.3 Å) | | Cite: | An ensemble of interconverting conformations of the elemental paused transcription complex creates regulatory options.
Proc.Natl.Acad.Sci.USA, 120, 2023
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8EG8
 | | Cryo-EM structure of consensus elemental paused elongation complex with a folded TL | | Descriptor: | CHAPSO, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | | Authors: | Kang, J.Y, Chen, J, Llewellyn, E, Landick, R, Darst, S.A. | | Deposit date: | 2022-09-12 | | Release date: | 2023-03-01 | | Last modified: | 2024-06-19 | | Method: | ELECTRON MICROSCOPY (3.3 Å) | | Cite: | An ensemble of interconverting conformations of the elemental paused transcription complex creates regulatory options.
Proc.Natl.Acad.Sci.USA, 120, 2023
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8EGB
 | | Cryo-EM structure of consensus elemental paused elongation complex with an unfolded TL | | Descriptor: | CHAPSO, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | | Authors: | Kang, J.Y, Chen, J, Llewellyn, E, Landick, R, Darst, S.A. | | Deposit date: | 2022-09-12 | | Release date: | 2023-03-01 | | Last modified: | 2024-06-19 | | Method: | ELECTRON MICROSCOPY (3.8 Å) | | Cite: | An ensemble of interconverting conformations of the elemental paused transcription complex creates regulatory options.
Proc.Natl.Acad.Sci.USA, 120, 2023
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8EG7
 | | Cryo-EM structure of pre-consensus elemental paused elongation complex | | Descriptor: | CHAPSO, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | | Authors: | Kang, J.Y, Chen, J, Llewellyn, E, Landick, R, Darst, S.A. | | Deposit date: | 2022-09-11 | | Release date: | 2023-03-01 | | Last modified: | 2024-06-19 | | Method: | ELECTRON MICROSCOPY (3.2 Å) | | Cite: | An ensemble of interconverting conformations of the elemental paused transcription complex creates regulatory options.
Proc.Natl.Acad.Sci.USA, 120, 2023
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8EHA
 | | Cryo-EM structure of his-elemental paused elongation complex with a folded TL and a rotated RH-FL (out) | | Descriptor: | CHAPSO, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | | Authors: | Kang, J.Y, Chen, J, Llewellyn, E, Landick, R, Darst, S.A. | | Deposit date: | 2022-09-14 | | Release date: | 2023-03-01 | | Last modified: | 2024-06-19 | | Method: | ELECTRON MICROSCOPY (3.7 Å) | | Cite: | An ensemble of interconverting conformations of the elemental paused transcription complex creates regulatory options.
Proc.Natl.Acad.Sci.USA, 120, 2023
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8EH8
 | | Cryo-EM structure of his-elemental paused elongation complex with a folded TL and a rotated RH-FL (1) | | Descriptor: | CHAPSO, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | | Authors: | Kang, J.Y, Chen, J, Llewellyn, E, Landick, R, Darst, S.A. | | Deposit date: | 2022-09-13 | | Release date: | 2023-03-01 | | Last modified: | 2024-06-19 | | Method: | ELECTRON MICROSCOPY (3.4 Å) | | Cite: | An ensemble of interconverting conformations of the elemental paused transcription complex creates regulatory options.
Proc.Natl.Acad.Sci.USA, 120, 2023
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8EH9
 | | Cryo-EM structure of his-elemental paused elongation complex with a folded TL and a rotated RH-FL (2) | | Descriptor: | CHAPSO, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | | Authors: | Kang, J.Y, Chen, J, Llewellyn, E, Landick, R, Darst, S.A. | | Deposit date: | 2022-09-13 | | Release date: | 2023-03-01 | | Last modified: | 2024-06-19 | | Method: | ELECTRON MICROSCOPY (3.9 Å) | | Cite: | An ensemble of interconverting conformations of the elemental paused transcription complex creates regulatory options.
Proc.Natl.Acad.Sci.USA, 120, 2023
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8F1I
 | | SigN RNA polymerase early-melted intermediate bound to mismatch fragment dhsU36mm1 (-12T) | | Descriptor: | DNA (36-MER), DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | | Authors: | Mueller, A.U, Chen, J, Darst, S.A. | | Deposit date: | 2022-11-05 | | Release date: | 2023-04-05 | | Last modified: | 2024-06-19 | | Method: | ELECTRON MICROSCOPY (3 Å) | | Cite: | A general mechanism for transcription bubble nucleation in bacteria.
Proc.Natl.Acad.Sci.USA, 120, 2023
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8F1J
 | | SigN RNA polymerase early-melted intermediate bound to mismatch DNA fragment dhsU36mm2 (-12A) | | Descriptor: | DNA (36-MER), DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | | Authors: | Mueller, A.U, Chen, J, Darst, S.A. | | Deposit date: | 2022-11-05 | | Release date: | 2023-04-05 | | Last modified: | 2024-06-19 | | Method: | ELECTRON MICROSCOPY (2.6 Å) | | Cite: | A general mechanism for transcription bubble nucleation in bacteria.
Proc.Natl.Acad.Sci.USA, 120, 2023
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8F1K
 | | SigN RNA polymerase early-melted intermediate bound to full duplex DNA fragment dhsU36 (-12T) | | Descriptor: | DNA (36-MER), DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ... | | Authors: | Mueller, A.U, Chen, J, Darst, S.A. | | Deposit date: | 2022-11-05 | | Release date: | 2023-04-05 | | Last modified: | 2024-06-19 | | Method: | ELECTRON MICROSCOPY (2.8 Å) | | Cite: | A general mechanism for transcription bubble nucleation in bacteria.
Proc.Natl.Acad.Sci.USA, 120, 2023
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4L6Y
 | | Structure of the microtubule associated protein PRC1 (Protein Regulator of Cytokinesis 1) | | Descriptor: | Protein regulator of cytokinesis 1 | | Authors: | Subramanian, R, Ti, S, Tan, L, Darst, S.A, Kapoor, T.M. | | Deposit date: | 2013-06-13 | | Release date: | 2013-07-17 | | Last modified: | 2023-09-20 | | Method: | X-RAY DIFFRACTION (3.3015 Å) | | Cite: | Marking and Measuring Single Microtubules by PRC1 and Kinesin-4.
Cell(Cambridge,Mass.), 154, 2013
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