6BY7
Folding DNA into a lipid-conjugated nano-barrel for controlled reconstitution of membrane proteins
This is a non-PDB format compatible entry.
Summary for 6BY7
| Entry DOI | 10.2210/pdb6by7/pdb |
| EMDB information | 7304 |
| Descriptor | DNA (52-MER), DNA (5'-D(*TP*AP*TP*TP*AP*GP*CP*GP*AP*GP*AP*TP*GP*GP*TP*TP*TP*TP*AP*TP*TP*AP*CP*A)-3'), DNA (4346-MER), ... (118 entities in total) |
| Functional Keywords | dna nano-barrel dna origami dna assembly, dna |
| Biological source | synthetic construct More |
| Total number of polymer chains | 118 |
| Total formula weight | 2715216.26 |
| Authors | |
| Primary citation | Dong, Y.,Chen, S.,Zhang, S.,Sodroski, J.,Yang, Z.,Liu, D.,Mao, Y. Folding DNA into a Lipid-Conjugated Nanobarrel for Controlled Reconstitution of Membrane Proteins. Angew. Chem. Int. Ed. Engl., 57:2072-2076, 2018 Cited by PubMed Abstract: Building upon DNA origami technology, we introduce a method to reconstitute a single membrane protein into a self-assembled DNA nanobarrel that scaffolds a nanodisc-like lipid environment. Compared with the membrane-scaffolding-protein nanodisc technique, our approach gives rise to defined stoichiometry, controlled sizes, as well as enhanced stability and homogeneity in membrane protein reconstitution. We further demonstrate potential applications of the DNA nanobarrels in the structural analysis of membrane proteins. PubMed: 29266648DOI: 10.1002/anie.201710147 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (7.5 Å) |
Structure validation
Download full validation report
