5HDD
Femtosecond Structural Dynamics Drives the Trans/Cis Isomerization in Photoactive Yellow Protein: 800 fs to 1200 fs Structure
Summary for 5HDD
| Entry DOI | 10.2210/pdb5hdd/pdb |
| Related | 2PHY 5HD3 5HD5 5HDC 5HDS |
| Descriptor | Photoactive yellow protein (2 entities in total) |
| Functional Keywords | photoreceptor cis trans isomerization trans cis isomerization free electron laser, signaling protein |
| Biological source | Halorhodospira halophila |
| Total number of polymer chains | 1 |
| Total formula weight | 14034.72 |
| Authors | Pande, K.,Tenboer, J.,Schmidt, M. (deposition date: 2016-01-05, release date: 2016-05-25, Last modification date: 2023-11-15) |
| Primary citation | Pande, K.,Hutchison, C.D.,Groenhof, G.,Aquila, A.,Robinson, J.S.,Tenboer, J.,Basu, S.,Boutet, S.,DePonte, D.P.,Liang, M.,White, T.A.,Zatsepin, N.A.,Yefanov, O.,Morozov, D.,Oberthuer, D.,Gati, C.,Subramanian, G.,James, D.,Zhao, Y.,Koralek, J.,Brayshaw, J.,Kupitz, C.,Conrad, C.,Roy-Chowdhury, S.,Coe, J.D.,Metz, M.,Xavier, P.L.,Grant, T.D.,Koglin, J.E.,Ketawala, G.,Fromme, R.,Srajer, V.,Henning, R.,Spence, J.C.,Ourmazd, A.,Schwander, P.,Weierstall, U.,Frank, M.,Fromme, P.,Barty, A.,Chapman, H.N.,Moffat, K.,van Thor, J.J.,Schmidt, M. Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein. Science, 352:725-729, 2016 Cited by PubMed Abstract: A variety of organisms have evolved mechanisms to detect and respond to light, in which the response is mediated by protein structural changes after photon absorption. The initial step is often the photoisomerization of a conjugated chromophore. Isomerization occurs on ultrafast time scales and is substantially influenced by the chromophore environment. Here we identify structural changes associated with the earliest steps in the trans-to-cis isomerization of the chromophore in photoactive yellow protein. Femtosecond hard x-ray pulses emitted by the Linac Coherent Light Source were used to conduct time-resolved serial femtosecond crystallography on photoactive yellow protein microcrystals over a time range from 100 femtoseconds to 3 picoseconds to determine the structural dynamics of the photoisomerization reaction. PubMed: 27151871DOI: 10.1126/science.aad5081 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.6 Å) |
Structure validation
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