CiCLE workshop: sample evaluation for cryo-EM
Outline：For obtaining high-resolution structures by single particle cryo-EM suitable sample evaluation and sample preparation is essential.
An important aspect of sample evaluation is the effective use of negative stain EM of the purified target protein.
In this workshop lectures and hands-on practice workshops will provide an introduction to the use of negative stain EM observation of protein sample
by conventional transmission electron microscopy (TEM) for the use in cryo-EM based structure determination.
January 6 and 7, 2020 (Each participant attends one of the two days.)
Institute for Protein Research (IPR), Osaka University
Christoph Gerle, Genji Kurisu（IPR, Osaka University）
- Number of Participants
About five persons for each day. In total ten persons.
When more than 10 people apply, we would like to select participants, considering reasons of application and the order of application.
Persons who plan to use electron microscopy have priorities.
- Where we will meet
9:00 AM at the 1F Lecture Hall in IPR
- Japanese and English
- Contact us
PDBj Contact Us
Program：January 6 or January 7, 2020
(The programs of Jan 6 and Jan 7 are the same.)
AM: Lectures [1F Lecture Hall]
- General introduction（Kurisu）
- 9:30 - 10:50:
- Electron microcopy（Gerle）
- 11:00- 12:00:
- How to make and evaluate sample for EM（Gerle）
PM：Practices (Gerle, Misumi)
- 13:30 - 15:00:
- Making grids [3F Kurisu Lab]
- 15:10 - 17:00:
- Observing negative-staining samples using TEM H-7650
[1F TEM room in Research Center for the State-of-the-Art Functional Protein Analysis]
Please fill in application form.
December 20, 2019