[wwPDB] Improved OneDep Support for 3D ED/MicroED
With the recent advancement of 3D Microcrystal Electron Diffraction (3D ED/MicroED), the number of Electron Crystallography structures deposited in the Protein Data Bank (PDB) has increased and expect to continue growing rapidly. This growth has highlighted the community need to improve PDB data deposition and representation to support this technology.
Since 2023, wwPDB has been working with 3D ED/MicroED community experts and mmCIF Working Group to develop a new data model as an extension of the PDBx/mmCIF dictionary. Following community recommendations, the metadata of 3D ED/MicroED structures are now collected using the Macromolecular Crystallography framework, with additional metadata for data collection and processing protocols pertains to this unique technology.
This new model of 3D ED/MicroED has been incorporated into wwPDB OneDep deposition system to collect improved metadata, including microcrystal preparation, continuous rotation data collection, and controlled electron fluence. Structure factors are now required for submission (rather than derived map files). These structure factors are then used to generate the corresponding validation reports.
Support has also been added for SerialED metadata collection through the PDBx/mmCIF Serial Crystallography extension.
In the near future, existing Electron Crystallography entries in the PDB will be remediated to conform to this data model.
Example data collected for X-ray, 3D ED/MicroED, and 3DEM structure solution procedures
[ wwPDB News ]
