9UVS

Crystal Structure of transaminase from Phaeobacter porticola

Summary for 9UVS

• Entry DOI: 10.2210/pdb9uvs/pdb
• Descriptor: Aminotransferase class-III (2 entities in total)
• Functional Keywords: transaminase, phaeobacter porticola, transferase
• Biological source: Phaeobacter porticola
• Total number of polymer chains: 2
• Total formula weight: 100715.52

Authors

Heo, Y.-S. (deposition date: 2025-05-11, release date: 2026-04-29)

Primary citation

Khobragade, T.P.,Giri, P.,Patil, M.D.,Joo, S.,Cho, S.,Kim, Y.,Ghosh, R.,Jeong, S.,Maeng, M.,Song, M.H.,Park, J.M.,Lee, E.H.,Keum, Y.S.,Kang, T.J.,Heo, Y.S.,Yun, H.
Total Biocatalytic Synthesis of Capsaicinoids Using Ferulic Acid: A Versatile Two-Step Strategy for Natural Product Diversification.
Angew.Chem.Int.Ed.Engl., 64:e202514504-e202514504, 2025

PubMed Abstract: The wide-ranging application of capsaicinoids, the active compounds in chili peppers, has driven increasing interest in the development of sustainable production strategies. However, capsaicinoid synthesis remains a challenge. The objective of this pioneering study is to report the total biocatalytic synthesis of structurally diverse capsaicinoids from bio-based ferulic acids. An X-ray crystallographic study elucidated the structural basis for the exceptional potential of a novel transaminase from Phaeobacter porticola (PPTA) to transform the highest ever reported concentration of vanillin (100-200 mM) to vanillylamine, with >99% conversion and modest conversion ranging from 48% to 79% for 300 to 500 mM substrate. Using PPTA in tandem with phenolic acid decarboxylase (PAD) and aromatic dioxygenase (ADO) further enabled the direct synthesis of vanillylamine from ferulic acid with >99% conversion. Furthermore, the integration of a multi-enzymatic cascade with carboxylic acid reductases (CARs) successfully synthesized structurally diverse capsaicinoids via amide bond formation between vanillylamine and free fatty acids, with excellent conversions ranging from 72% to >88%. A 50-mM enzymatic reaction afforded 95% and 80% conversion of vanillylamine and capsaicin, respectively.

PubMed: 41055281
DOI: 10.1002/anie.202514504

Experimental method

X-RAY DIFFRACTION (1.73 Å)