9U8H

Crystal structure of Leminorella grimontii GatC in the absence of D-xylose

9U8H の概要

• エントリーDOI: 10.2210/pdb9u8h/pdb
• 関連するPDBエントリー: 9U82 9U84 9U8E
• 分子名称: PTS galactitol transporter subunit IIC, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate (2 entities in total)
• 機能のキーワード: pts, transporter, xylose, transport protein
• 由来する生物種: Leminorella grimontii
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 102295.36

構造登録者

Takahashi, Y.S.,Kohga, H.,Yamamoto, K.,Takahashi, J.F.,Tanaka, Y.,Ichikawa, M.,Miyazaki, R.,Tsukazaki, T. (登録日: 2025-03-26, 公開日: 2025-10-15, 最終更新日: 2026-03-04)

主引用文献

Takahashi, Y.S.,Kohga, H.,Chek, M.F.,Yamamoto, K.,Takahashi, J.F.,Shigematsu, H.,Tanaka, Y.,Ichikawa, M.,Miyazaki, R.,Hakoshima, T.,Tsukazaki, T.
Structural basis of a GatC ortholog transporter in the bacterial phosphotransferase system.
Febs Lett., 599:2377-2387, 2025

PubMed Abstract: The bacterial phosphotransferase system (PTS) mediates the uptake of specific carbohydrates via IIC transporters. Here, we report the crystal and cryo-electron microscopy (cryo-EM) structures of Leminorella grimontii galactitol-specific PTS enzyme IIC component (LgGatC), which is implicated in D-xylose uptake and belongs to the ascorbate-galactitol (AG) superfamily of IIC proteins. These structures, determined in the presence and absence of D-xylose, capture the transporter in an outward-facing conformation. A homology model of an inward-facing state, constructed based on these structures, supports an elevator-like transport mechanism. These findings provide structural insights into substrate recognition by GatC and offer a framework for understanding sugar transport in PTS IIC proteins.

PubMed: 40878824
DOI: 10.1002/1873-3468.70135

実験手法

X-RAY DIFFRACTION (3.74 Å)