9TMP

Crystal Structure of Native Cytochrome b562 in complex with the synthetic anti-BRIL antibody BAG2.

9TMP の概要

• エントリーDOI: 10.2210/pdb9tmp/pdb
• 分子名称: Soluble cytochrome b562, anti-BRIL antibody - heavy chain, anti-BRIL antibody - light chain, ... (5 entities in total)
• 機能のキーワード: cytochrome, fab, bril, protein binding
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 62502.62

構造登録者

Cooper, B.F.,Isom, G.L. (登録日: 2025-12-15, 公開日: 2026-03-25, 最終更新日: 2026-04-01)

主引用文献

Cooper, B.F.,Isom, G.L.
Optimized bacterial expression of a synthetic BRIL antibody.
Acta Crystallogr.,Sect.F, 2026

PubMed Abstract: The use of monoclonal fragments antigen binding (Fabs) is a prevalent methodology facilitating protein structure determination via both crystallography and cryo-EM. The development of a synthetic Fab against the BRIL domain improved the accessibility of this approach, providing a general fiducial applicable to any protein of interest via the simple curation of a BRIL fusion protein. Here, we document the generation of a T7 Express ΔcybC strain allowing contaminant-free bacterial expression of the synthetic anti-BRIL Fab BAG2. We also report the crystal structure of BAG2 in complex with native cytochrome b, a complex arising from expression in canonical Escherichia coli strains.

PubMed: 41842660
DOI: 10.1107/S2053230X26001548

実験手法

X-RAY DIFFRACTION (2.65 Å)