9TBF

Cryo-EM structure of the light-driven sodium pump ErNaR in the monomeric form in the O2 state

9TBF の概要

• エントリーDOI: 10.2210/pdb9tbf/pdb
• 関連するPDBエントリー: 9TBD 9TBE
• EMDBエントリー: 55761 55765 55766 55769
• 分子名称: Bacteriorhodopsin-like protein, EICOSANE, DODECYL-BETA-D-MALTOSIDE, ... (5 entities in total)
• 機能のキーワード: rhodopsin, ernar, sodium pump, phototransduction, membrane protein
• 由来する生物種: Erythrobacter
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35318.22

構造登録者

Haubner, M.,Williams, H.M.,Hruby, J.,Straub, M.S.,Guskov, A.,Kovalev, K.,Drabbels, M.,Lorenz, U.J. (登録日: 2025-11-19, 公開日: 2025-12-17)

主引用文献

Haubner, M.,Williams, H.M.,Hruby, J.,Straub, M.S.,Guskov, A.,Kovalev, K.,Drabbels, M.,Lorenz, U.J.
Microsecond Time-Resolved Cryo-EM Based on Jet Vitrification.
Biorxiv, 2025

PubMed Abstract: Understanding and ultimately predicting the function of proteins is one of the frontiers in structural biology. This will only be possible if it becomes feasible to routinely observe proteins on the fast timescales on which they perform their tasks. Recently, laser flash melting and revitrification experiments have improved the time resolution of cryo-electron microscopy (cryo-EM) to microseconds, rendering it fast enough to observe the domain motions of proteins that are frequently linked to function. However, observations have been limited to a time window of just a few hundred microseconds. Here, we introduce time-resolved cryo-EM experiments based on jet vitrification that combine microsecond resolution with an observation window of up to seconds. We use a short laser pulse to initiate protein dynamics, and as they unfold, vitrify the sample with a jet of a liquid cryogen to arrest the dynamics at that point in time. We demonstrate that our approach affords near-atomic spatial resolution and a time resolution of 21 μs. This allows us to observe the photoinduced dynamics of the light-driven sodium pump NaR on the microsecond to millisecond timescale. Our experiments significantly expand the ability of cryo-EM to observe protein dynamics across multiple timescales.

PubMed: 41332690
DOI: 10.1101/2025.11.21.689681

実験手法

ELECTRON MICROSCOPY (3.3 Å)