9SRK

Structure of collectin-11 (CL-11) carbohydrate-recognition domain in complex with L-fucose

Summary for 9SRK

• Entry DOI: 10.2210/pdb9srk/pdb
• Descriptor: Collectin-11, CALCIUM ION, alpha-L-fucopyranose, ... (6 entities in total)
• Functional Keywords: innate immunity, complement, lectin, immune system
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 30245.90

Authors

Wallis, R.,Alrehaili, A.F.M.,Sacks, S.H.,klavinskis, L.S. (deposition date: 2025-09-24, release date: 2025-11-05)

Primary citation

Polycarpou, A.,Wagner-Gamble, T.,Greenlaw, R.,O'Neill, L.,Kanabar, V.,Alrehaili, A.,Jeon, Y.,Baker, J.,Bafadhel, M.,Khan, H.,Malim, M.H.,Romano, M.,Farrar, C.A.,Smolarek, D.,Martinez-Nunez, R.,Doores, K.J.,Wallis, R.,Klavinskis, L.S.,Sacks, S.H.
Glycan recognition by collectin-11 drives SARS-CoV-2 infectivity and membrane injury of respiratory epithelial cells.
Proc.Natl.Acad.Sci.USA, 122:e2521209122-e2521209122, 2025

PubMed Abstract: SARS-CoV-2 respiratory-tract infection affects both vaccinated and unvaccinated persons suggesting factors besides adaptive immunity are operative. We investigated the role of collectin-11 (CL-11), an epithelial-secreted carbohydrate-binding lectin that drives innate immunity and eliminates pathogens by complement activation. SARS-CoV-2, despite binding CL-11 to activate complement, was resistant to lysis. Remarkably, opsonization by CL-11 enhanced virus production by infected respiratory epithelial cells independently of complement. Furthermore, infected cells expressing SARS-CoV-2 spike protein displayed enhanced vulnerability to CL-11 binding and membrane attack by complement. The mechanism of enhanced infectivity was ablated in the presence of L-fucose, which occupied the extended carbohydrate-binding cleft of CL-11 in a crystallographic analysis of complexes between L-fucose and CL-11. Our study suggests pathogenicity of SARS-CoV-2 is related to complement-resistance together with enhanced infectivity and injury of respiratory epithelial cells mediated by locally released CL-11.

PubMed: 41134631
DOI: 10.1073/pnas.2521209122

Experimental method

X-RAY DIFFRACTION (1.8 Å)