9SOB

Structural Model of the Nuclear Pore Complex in Arabidopsis thaliana

これはPDB形式変換不可エントリーです。

9SOB の概要

• エントリーDOI: 10.2210/pdb9sob/pdb
• EMDBエントリー: 54657
• 分子名称: Nuclear pore complex protein NUP160, Nuclear pore complex protein NUP205, Nuclear pore complex protein NUP93A, ... (24 entities in total)
• 機能のキーワード: nuclear pore complex, nuclear transport, nuclear-cytoplasmic transport, nuclear protein
• 由来する生物種: Arabidopsis thaliana (thale cress); 詳細
• タンパク質・核酸の鎖数: 70
• 化学式量合計: 5802829.11

構造登録者

Obarska-Kosinska, A.,Sanchez Carrillo, I.B.,Hoffmann, P.C.,Fourcassie, V.,Beck, M.,Germain, H. (登録日: 2025-09-12, 公開日: 2025-11-05, 最終更新日: 2025-12-03)

主引用文献

Sanchez Carrillo, I.B.,Hoffmann, P.C.,Obarska-Kosinska, A.,Fourcassie, V.,Beck, M.,Germain, H.
In situ architecture of the nuclear pore complex of the higher plant Arabidopsis thaliana.
Nat.Plants, 11:2368-2380, 2025

PubMed Abstract: The nucleus is enclosed by the nuclear envelope, which contains nuclear pore complexes (NPCs). While NPCs have been well studied in vertebrates, yeast and algae, in situ structural data for higher plants is lacking. Here we show that individual nucleoporins of Arabidopsis thaliana and humans exhibit high structural similarity. We report an in situ NPC structure of higher plants, derived from A. thaliana root protoplasts using cryo-electron tomography, subtomogram averaging and homology-based integrative modelling. We present the AtNPC model based on predictions of A. thaliana nucleoporins (NUPs), supported by mass spectrometry. Here the AtNPC scaffold contains one Y-complex ring at the cytosolic and two at the nuclear ring. The AtNPC contains prominent NUP155 connector elements that are conserved in human NPCs but not in Chlamydomonas reinhardtii NPCs. Our model suggests that the ELYS homologue HOS1 plays an important role in the head-to-tail connection of Y-complexes in AtNPCs.

PubMed: 41174229
DOI: 10.1038/s41477-025-02138-y

実験手法

ELECTRON MICROSCOPY (35 Å)