9RP3

Structure of Avast type 5 activator (Gp316, JADA jumbophage protein)

9RP3 の概要

• エントリーDOI: 10.2210/pdb9rp3/pdb
• EMDBエントリー: 54139
• 分子名称: Gp316/JADA (1 entity in total)
• 機能のキーワード: jumbophage, gp316, jada, avast5, dimer, unknown function
• 由来する生物種: Pseudomonas phage vB_PA32_GUMS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 160422.69

構造登録者

Pacesa, M.,Muralidharan, A.,Brouns, S.J.J. (登録日: 2025-06-23, 公開日: 2025-07-02, 最終更新日: 2026-03-04)

主引用文献

Muralidharan, A.,Costa, A.R.,Fierlier, D.,van den Berg, D.F.,van den Bossche, H.,Zoumaro-Djayoon, A.D.,Rodriguez-Molina, A.,Pabst, M.,Pacesa, M.,Correia, B.E.,Brouns, S.J.J.
Molecular basis for anti-jumbo phage immunity by AVAST type 5.
Mol.Cell, 86:740-, 2026

PubMed Abstract: Jumbo phages protect their genomes from DNA-sensing bacterial defense systems by enclosing them within vesicles and nucleus-like compartments. Very little is known about defense systems specialized to counter these phages. Here, we show that AVAST type 5 (Avs5) systems, part of the signal transduction ATPases of numerous domains (STAND) superfamily, confer conserved immunity against jumbo phages. Using fluorescence microscopy and biotin proximity labeling, we demonstrate that Avs5 localizes to early infection vesicles, where it senses an essential, early-expressed phage protein named JADA (Jumbo phage Avs5 Defense Activator). Recognition of phage infection triggers the Sir2-like effector domain of Avs5 across three Avs5 clades, resulting in rapid NAD hydrolysis, disruption of phage nucleus formation, and arrest of infection. These findings reveal a spatially coordinated bacterial immune strategy that targets an early vulnerability in jumbo phage infection.

PubMed: 41653918
DOI: 10.1016/j.molcel.2026.01.004

実験手法

ELECTRON MICROSCOPY (2.1 Å)