9QH1

ATP-bound wild type Wzm-Wzt from Mycobacterium abscessus in LMNG

9QH1 の概要

• エントリーDOI: 10.2210/pdb9qh1/pdb
• 関連するPDBエントリー: 9QFX 9QGU 9QHJ 9QHK 9QHV 9QHW 9QHX
• EMDBエントリー: 53123 53148 53152
• 分子名称: Probable O-antigen/lipopolysaccharide transport integral membrane protein ABC transporter RfbD, Probable o-antigen/lipopolysaccharide transport ATP-binding protein ABC transporter RfbE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: abc transporter type v abc transporter arabinogalactan precursor lipid-linked galactan exporter, membrane protein
• 由来する生物種: Mycobacteroides abscessus; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 129089.41

構造登録者

Garaeva, A.A.,Fabianova, V.,Savkova, K.,Huszar, S.,Xue, X.,Lowary, T.L.,Mikusova, K.,Seeger, M.A. (登録日: 2025-03-17, 公開日: 2026-03-11, 最終更新日: 2026-04-08)

主引用文献

Garaeva, A.A.,Fabianova, V.,Savkova, K.,Huszar, S.,Xue, X.,Lowary, T.L.,Mikusova, K.,Seeger, M.A.
Structural basis of lipid-linked galactan export by the mycobacterial ABC transporter Wzm-Wzt.
Nat Commun, 17:-, 2026

PubMed Abstract: Mycobacteria, including Mycobacterium tuberculosis, possess a unique cell envelope containing arabinogalactan, a heteropolysaccharide critical for cell wall integrity and target of several tuberculosis drugs. The cytosolic precursor of arabinogalactan, lipid-linked galactan (LLG), is translocated across the plasma membrane by the essential ABC transporter Wzm-Wzt through a molecular mechanism that is poorly understood. Here, we present a series of cryo-EM structures of Wzm-Wzt from Mycobacterium abscessus, representing different conformations of the transport cycle. Conserved residues lining the proposed LLG translocation pathway were investigated by three orthologous functional assays, revealing that the cytosolic gate helix (GH) plays a key functional role in polysaccharide transport. Our data suggests that the hydrophobic polyprenyl-moiety is translocated first, followed by the galactan-polysaccharide, which requires Wzm-Wzt to open a continuous channel through which the sugar chain is ratcheted at the expense of ATP hydrolysis. Our results provide a rational basis for the development of drugs that inhibit mycobacterial cell wall biosynthesis.

PubMed: 41839883
DOI: 10.1038/s41467-026-70429-9

実験手法

ELECTRON MICROSCOPY (3.44 Å)