9PR8

Crystal Structure of the Clostridiodes difficile CspC-CspA heterodimer.

9PR8 の概要

• エントリーDOI: 10.2210/pdb9pr8/pdb
• 分子名称: Germination-specific protease (3 entities in total)
• 機能のキーワード: pseudoproteases, heterodimer, signaling proteins, signaling protein
• 由来する生物種: Clostridia; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 245133.19

構造登録者

Heldwein, E.E.,Gonzalez-Del Pino, G.L. (登録日: 2025-07-23, 公開日: 2026-01-21, 最終更新日: 2026-02-18)

主引用文献

McNellis, M.E.,Gonzalez-Del Pino, G.,Serrano-Jimenez, J.A.,Forster, E.R.,Stoica, A.I.,Heldwein, E.E.,Shen, A.
The CspC:CspA heterodimer transduces germinant and co-germinant signals during Clostridioides difficile spore germination.
Plos Biol., 24:e3003610-e3003610, 2026

PubMed Abstract: The clinically significant pathogen Clostridioides difficile lacks the transmembrane nutrient germinant receptors conserved in almost all spore-forming bacteria. Instead, C. difficile initiates spore germination using a unique mechanism that requires two signals: a bile acid germinant and a co-germinant, which can be either an amino acid or a divalent cation. While two soluble pseudoproteases, CspC and CspA, were initially identified as the germinant and co-germinant receptors, respectively, in C. difficile, we previously identified residues in an unstructured region of CspC that regulate the sensitivity of C. difficile spores to both signals. However, the mechanism by which CspC transduces these signals remained unclear. Here, we demonstrate that CspC forms a stable complex with CspA and determine the crystal structure of the CspC:CspA heterodimer. The structure reveals extensive interactions along the binding interface, including direct interactions between the unstructured region of CspC and CspA. Using structure-function analyses, we identify CspC:CspA interactions that regulate the sensitivity of C. difficile spores to germinant signals and show that CspA regulates the response of C. difficile to not only co-germinant but also germinant signals. While we show that CspA can form a homodimer and determine its crystal structure, CspA homodimerization appears unimportant for C. difficile spore germination. Collectively, our analyses establish the CspC:CspA heterodimer, rather than its individual constituents, as a critical signaling node for sensing both germinant and co-germinant signals. They also suggest a new mechanistic model for how C. difficile transduces germinant signals, which could guide the development of therapeutics against this important pathogen.

PubMed: 41628221
DOI: 10.1371/journal.pbio.3003610

実験手法

X-RAY DIFFRACTION (3.35 Å)