9PH0

Crystal structure of MAR1 from Gelatoporia subvermispora

9PH0 の概要

• エントリーDOI: 10.2210/pdb9ph0/pdb
• 分子名称: MAR1, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: mar1, white-rot fungi, lignin, oxidoreductase
• 由来する生物種: Gelatoporia subvermispora B
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 178779.18

構造登録者

Mathews, I.I.,Kuatsjah, E.,Schwartz, A.,Sarangi, R.,McGeehan, J.E.,Salvachua, D. (登録日: 2025-07-08, 公開日: 2026-06-17)

主引用文献

Schwartz, A.,Kuatsjah, E.,Mathews, I.I.,Mitchell, H.D.,Purvine, S.O.,Eder, E.K.,Hoyt, D.W.,Markillie, L.M.,Burnet, M.C.,Woodworth, S.P.,Ingraham, M.A.,Ramirez, K.J.,Monteiro, L.M.O.,Sarangi, R.,McGeehan, J.E.,Salvachua, D.
Catabolism of lignin-related methoxylated compounds in white-rot fungi utilizes non-canonical oxidoreductases.
Cell Rep, 45:117428-117428, 2026

PubMed Abstract: White-rot fungi (WRF) are the most effective lignin-degrading organisms in nature. Lignin is a highly methoxylated plant biopolymer, yet the pathways WRF use to metabolize methoxylated aromatic monomeric compounds as carbon sources remain unidentified. Using systems biology, we elucidate the intracellular catabolism of vanillate-a monomethoxylated aromatic compound-by Gelatoporia subvermispora and Trametes versicolor. We identify and biochemically validate a four-enzyme pathway that converts vanillate into β-ketoadipate, which enters central carbon metabolism. Unlike bacteria, which demethylate vanillate before ring-cleavage by intradiol dioxygenases, WRF employ oxidative decarboxylation followed by extradiol dioxygenase-mediated cleavage. A previously uncharacterized hydrolase is also shown to catalyze the terminal step of this pathway. Biochemical and structural approaches reveal non-canonical enzymes, including a highly substrate-specific extradiol dioxygenase and a metal-free, promiscuous reductase that acts on both methoxylated and non-methoxylated intermediates. These findings highlight distinct fungal strategies for aromatic degradation, offering insights into lignin valorization and wood decay mechanisms.

PubMed: 42217187
DOI: 10.1016/j.celrep.2026.117428

実験手法

X-RAY DIFFRACTION (1.79 Å)