9P6H

Structure of P. gingivalis PorK and PorN complexes from cryo electron microscopy

9P6H の概要

• エントリーDOI: 10.2210/pdb9p6h/pdb
• EMDBエントリー: 71309
• 分子名称: Gliding motility protein GldN, Lipoprotein, putative, 2-acetamido-2-deoxy-beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-galactopyranose-(1-4)-beta-L-fucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-alpha-D-glucopyranuronic acid-(1-2)-[alpha-L-rhamnopyranose-(1-4)]alpha-D-mannopyranose, ... (6 entities in total)
• 機能のキーワード: t9ss, porphyromonas gingivalis, transport protein
• 由来する生物種: Porphyromonas gingivalis W83; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 84764.89

構造登録者

Hanssen, E.,Morton, C.,Gorasia, D.G.,Veith, P.D.,Reynolds, E.C. (登録日: 2025-06-19, 公開日: 2025-07-16, 最終更新日: 2025-09-03)

主引用文献

Gorasia, D.G.,Hanssen, E.,Mudaliyar, M.,Morton, C.J.,Valimehr, S.,Seers, C.,Zhang, L.,Doyle, M.T.,Ghosal, D.,Veith, P.D.,Reynolds, E.C.
Insights into type IX secretion from PorKN cogwheel structure bound to PorG and attachment complexes.
Nat Commun, 16:7735-7735, 2025

PubMed Abstract: The Type IX Secretion System exports proteins across the outer membrane (OM) of bacteria in the Bacteroidota phylum, however, the mechanistic details remain unknown. In Porphyromonas gingivalis the core components of the multi-protein complex are the Sov translocon, Attachment Complexes (PorQ, U, V, Z), PorLM molecular motors and PorKN rings. Here, we present a ~ 3.5 Å cryo-EM structure of the periplasmic rings comprising 32-33 subunits each of PorK and PorN. Additionally, we show the presence of a critical disulfide bond between PorK and the OM protein PorG that is essential for protein secretion and demonstrate that the Attachment Complexes bind to, and are localized above, the PorKN rings. Overall, each ring resembles a cogwheel with PorN forming cog-like projections that we propose engage with the PorLM motor to drive the rotation of the PorKN cogwheel together with PorG and associated Attachment Complexes, thus providing the energy to complete protein secretion and the coordinated cell surface attachment of the secreted cargo.

PubMed: 40830366
DOI: 10.1038/s41467-025-63163-1

実験手法

ELECTRON MICROSCOPY (2.75 Å)