9OM4

Cryo-EM structure of filament form Acidithiobacillus caldus (Aca) short prokaryotic argonautes, HNH-associated (SPARHA) with gRNA and tDNA

Summary for 9OM4

• Entry DOI: 10.2210/pdb9om4/pdb
• EMDB information: 70606
• Descriptor: HNH endonuclease, Prokaryotic argonaute MID-PIWI family protein, RNA (5'-R(P*AP*UP*UP*UP*GP*GP*UP*UP*GP*CP*GP*GP*UP*GP*CP*UP*CP*U)-3'), ... (5 entities in total)
• Functional Keywords: sparha, prokaryotic argonaute, nuclease, immune system, immune system-dna-rna complex, immune system/dna/rna
• Biological source: Acidithiobacillus caldus; More
• Total number of polymer chains: 16
• Total formula weight: 458707.44

Authors

Murakami, K.S.,Narwal, M. (deposition date: 2025-05-13, release date: 2025-10-22)

• Primary citation: Murakami, K.S.,Narwal, M.,Kanevskaya, A.,Kulbachinskiy, A.; Target-induced Argonaute-HNH filaments confer bacterial immunity; To Be Published,

Experimental method

ELECTRON MICROSCOPY (3.38 Å)