9OGM

BG505 MD39.3 Env gp151 MPER nanodisc in complex with 10E8, BG18 and VRC01 Fabs (1x 10E8 Fab)

これはPDB形式変換不可エントリーです。

9OGM の概要

• エントリーDOI: 10.2210/pdb9ogm/pdb
• EMDBエントリー: 70471
• 分子名称: BG18 Fab heavy chain, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (18 entities in total)
• 機能のキーワード: mper, hiv-1, broadly neutralizing antibody, viral protein, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 17
• 化学式量合計: 601524.02

構造登録者

Rantalainen, K.,Ozorowski, G.,Gharpure, A.,Ward, A.B. (登録日: 2025-05-01, 公開日: 2025-07-30)

主引用文献

Rantalainen, K.,Liguori, A.,Ozorowski, G.,Flynn, C.,Steichen, J.M.,Swanson, O.,Madden, P.J.,Baboo, S.,Phulera, S.,Gharpure, A.,Lu, D.,Kalyuzhniy, O.,Skog, P.,Terada, S.,Shil, M.,Diedrich, J.K.,Georgeson, E.,Tingle, R.,Eskandarzadeh, S.,Lee, W.H.,Alavi, N.,Goodwin, D.,Kubitz, M.,Amirzehni, S.,Himansu, S.,Sok, D.,Lee, J.H.,Yates, J.R.,Paulson, J.C.,Crotty, S.,Schiffner, T.,Ward, A.B.,Schief, W.R.
Virus glycoprotein nanodisc platform for vaccine design.
Biorxiv, 2025

PubMed Abstract: Transmembrane glycoproteins of enveloped viruses are the targets of neutralizing antibodies and essential vaccine antigens. mRNA-LNP technology allows in situ production of transmembrane glycoproteins upon immunization, but biophysical characterization of transmembrane antigens and in vitro analysis of post-immunization antibody responses typically rely on soluble proteins. Here, we present a methodological platform for assembling transmembrane glycoprotein vaccine candidates into lipid nanodiscs. We demonstrate the utility of the nanodiscs in HIV membrane proximal external region (MPER)-targeting vaccine development by binding assays using surface plasmon resonance (SPR), ex vivo B cell sorting with fluorescence-activated cell sorting (FACS), and by determining the structure of a prototypical HIV MPER-targeting immunogen nanodisc in complex with three broadly neutralizing antibodies (bnAbs), including the MPER bnAb 10E8, to 3.5 Å by cryogenic electron microscopy (cryo-EM), providing a template for structure-based immunogen design for MPER. Overall, the platform offers a tool for accelerating the development of next-generation viral vaccines.

PubMed: 40666859
DOI: 10.1101/2025.05.02.651272

実験手法

ELECTRON MICROSCOPY (3.5 Å)