9O7M
Cryo-EM Structure of YfdQ Reveals a Widespread Novel Family of Bacteriophage-Associated Proteins with Shell-Like Assemblies
Summary for 9O7M
| Entry DOI | 10.2210/pdb9o7m/pdb |
| EMDB information | 70204 |
| Descriptor | YfdQ Protein (1 entity in total) |
| Functional Keywords | d18 symmetry, shell-like structure, duf2303, yfdq, viral protein |
| Biological source | Caudoviricetes |
| Total number of polymer chains | 36 |
| Total formula weight | 1060456.14 |
| Authors | |
| Primary citation | Merighi, D.G.S.,Pompeu, G.C.,Araujo, G.G.,Silva de Souza, A.,Sgro, G.G.,Farah, C.S.,Guzzo, C.R. Cryo-EM structure of YfdQ reveals a widespread family of bacteriophage-associated proteins with shell-like assemblies. Structure, 2026 Cited by PubMed Abstract: Bacteriophages are key players in microbial ecosystems with strong biotechnological potential, yet many phage-encoded proteins remain uncharacterized. We present the structure of the YfdQ complex from the Xanthomonas citri ΦXacm4-11 phage, a conserved DUF2303 protein encoded by double-stranded DNA bacteriophages of the Caudoviricetes class. Using cryo-electron microscopy, we report that native YfdQ assembles into a shell-like structure with D18 symmetry forming a ∼1.1 MegaDalton complex, a hollow interior and a unique equatorial β strand stitching oligomerization interface that holds together the two hemispheres. YfdQ homologs are widely distributed among phages infecting diverse bacterial hosts, suggesting a conserved biological role. YfdQ are found in prophages and cryptic phages. Our results suggest that heterologous expression of YfdQ in X. citri and in Escherichia coli allows the obtention of structurally heterogeneous YfdQ complexes, suggesting that its proper assembly into the shell-like structure may depend on still unidentified viral factors. PubMed: 42309054DOI: 10.1016/j.str.2026.05.009 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.9 Å) |
Structure validation
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