9N6H

2.54 A S.cerevisiae Chd1[L886G/L889G/L891G]-nucleosome 1:1 complex

9N6H の概要

• エントリーDOI: 10.2210/pdb9n6h/pdb
• 関連するPDBエントリー: 9N6I 9N6K
• EMDBエントリー: 49060 49061 49062 49063 49064
• 分子名称: DNA Tracking Strand, DNA Lagging Strand, Histone H4, ... (7 entities in total)
• 機能のキーワード: chromatin, chd1, remodeler, atp-dependent chromatin remodeler, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 283838.85

構造登録者

Nodelman, I.M.,Folkwein, H.J.,Armache, J.-P.,Bowman, G.D. (登録日: 2025-02-05, 公開日: 2025-06-11, 最終更新日: 2025-08-20)

主引用文献

Nodelman, I.M.,Folkwein, H.J.,Glime, W.S.,Armache, J.P.,Bowman, G.D.
A competitive regulatory mechanism of the Chd1 remodeler is integral to distorting nucleosomal DNA.
Nat.Struct.Mol.Biol., 32:1445-1455, 2025

PubMed Abstract: The Chd1 chromatin remodeler repositions nucleosomes into evenly spaced arrays, a characteristic of most eukaryotic genes. Here we show that the yeast Chd1 remodeler requires two activating segments to distort nucleosomal DNA into an A-form-like conformation, a critical first step in nucleosome sliding. As shown by cryo-electron microscopy, these two activating segments together pack against the ATPase motor, where they are poised to stabilize the central ATPase cleft. These activating elements contact the ATPase at locations that are incompatible with binding of NegC, an autoinhibitory segment located between the two activators. NegC inhibits sliding by antagonizing the activators through steric competition and constraining activator placement, giving rise to directional nucleosome sliding. Given that activator reinforcement of the ATPase cleft is needed for DNA distortion, this first step in remodeling appears to provide a natural checkpoint for regulation of chromatin remodeler activity.

PubMed: 40437259
DOI: 10.1038/s41594-025-01556-y

実験手法

ELECTRON MICROSCOPY (2.54 Å)