9MSA

Alpha-ketoisovalerate decarboxylase (Kivd) from Synechocystis sp. PCC 6803 with substitution S286T

9MSA の概要

• エントリーDOI: 10.2210/pdb9msa/pdb
• 分子名称: Alpha-ketoisovalerate decarboxylase, THIAMINE DIPHOSPHATE, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: synechocystis, isobutanol production, 3-methyl-1-butanol, alpha-ketoisovalerate decarboxylase, kivd, lyase
• 由来する生物種: Lactococcus lactis subsp. lactis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 245144.03

構造登録者

Begum, A.,Xie, H.,Gunn, L.H. (登録日: 2025-01-09, 公開日: 2025-08-13)

主引用文献

Xie, H.,Begum, A.,Gunn, L.H.,Lindblad, P.
Directed evolution of alpha-ketoisovalerate decarboxylase for improved isobutanol and 3-methyl-1-butanol production in cyanobacteria.
Biotechnol Biofuels Bioprod, 18:84-84, 2025

PubMed Abstract: Cyanobacteria are promising platforms for metabolic engineering to convert carbon dioxide into valuable fuels and chemicals, addressing both energy demands and global climate change. Among various fuels and chemicals, isobutanol (IB) and 3-methyl-1-butanol (3M1B) have gained increasing attention due to their superior physical properties, such as high energy density, low water solubility, and low hygroscopicity. Heterologously expressing α-ketoisovalerate decarboxylase (Kivd) in the unicellular cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis) enables microbial production of IB and 3M1B through the 2-keto acid pathway, with Kivd identified as a key bottleneck limiting production efficiency.

PubMed: 40745552
DOI: 10.1186/s13068-025-02687-6

実験手法

X-RAY DIFFRACTION (2.79 Å)