9MRH

Fluorescence lifetime-readout citrate sensor

9MRH の概要

• エントリーDOI: 10.2210/pdb9mrh/pdb
• 分子名称: Fluorescence lifetime-readout citrate sensor, CITRIC ACID, PENTAETHYLENE GLYCOL, ... (5 entities in total)
• 機能のキーワード: fluorescence lifetime, biosensor, citrate, fluorescent protein
• 由来する生物種: Aequorea
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 86895.76

構造登録者

Rosen, P.C.,Yellen, G.,Lim, D.C. (登録日: 2025-01-07, 公開日: 2025-11-12)

主引用文献

Rosen, P.C.,Fu, P.,Ferran, B.,Kim, E.,Brooks, D.J.,Lim, D.C.,Diaz-Garcia, C.M.,Yellen, G.
Activity-dependent citrate dynamics in neurons.
Proc.Natl.Acad.Sci.USA, 122:e2519902122-e2519902122, 2025

PubMed Abstract: Glycolytic enzymes sense metabolite levels to adapt rapidly to changing energy demands, but measuring the levels of these effectors with spatiotemporal precision in live cells has been challenging. We addressed this question in the context of neuronal depolarization, which activates glycolysis, focusing on the glycolysis inhibitor citrate. We engineered a pair of quantitative fluorescent biosensors for citrate that address several limitations (affinity, pH, Mg, and temperature) of existing citrate biosensors. Using two-photon fluorescence lifetime imaging, we found that free citrate in the cytosol of neurons in acute mouse brain slices declines two-to-threefold within seconds of neuronal activation and then returns to baseline over a few minutes. The stimulation-dependent citrate transient depends at least in part on the mitochondrial calcium uniporter. These types of live metabolite measurements are essential for achieving a nuanced understanding of the fast control of glycolysis.

PubMed: 41071660
DOI: 10.1073/pnas.2519902122

実験手法

X-RAY DIFFRACTION (2.37 Å)