9LK5
The structure of Lhcb8-C2S2 PSII-LHCII supercomplex from Arabidopsis thaliana
Summary for 9LK5
| Entry DOI | 10.2210/pdb9lk5/pdb |
| EMDB information | 63168 |
| Descriptor | Photosystem II protein D1, Photosystem II reaction center protein J, Photosystem II reaction center protein K, ... (39 entities in total) |
| Functional Keywords | photosystem ii, light harvesting, photoacclimation, stress-induced antennae, cryo-em, photosynthesis |
| Biological source | Arabidopsis thaliana (thale cress) More |
| Total number of polymer chains | 46 |
| Total formula weight | 1190931.24 |
| Authors | Zhou, Q.,Caferri, R.,Shan, J.Y.,Amelii, A.,Bassi, R.,Liu, Z.F. (deposition date: 2025-01-16, release date: 2025-06-18, Last modification date: 2025-08-13) |
| Primary citation | Caferri, R.,Zhou, Q.,Dall'Osto, L.,Amelii, A.,Shan, J.,Liu, Z.,Bassi, R. A stress-induced paralog of Lhcb4 controls the photosystem II functional architecture in Arabidopsis thaliana. Nat Commun, 16:6910-6910, 2025 Cited by PubMed Abstract: Photosystem II (PSII) is the pigment-protein complex catalysing light-induced water oxidation. In Arabidopsis thaliana, it includes three Lhcb4-6 proteins linking the core complex to peripheral trimeric antennae. While Lhcb5 and Lhcb6 are encoded by single genes, Lhcb4 is encoded by three isoforms: Lhcb4.1 and Lhcb4.2, constitutively expressed, and Lhcb4.3 (Lhcb8), which accumulates under prolonged abiotic stress. Lhcb8 substitutes for Lhcb4, preventing Lhcb6 accumulation and resulting in a smaller PSII with high quantum yield. Cryo-electron microscopy reveals that Lhcb8 has a shorter carboxy-terminal domain, lacks two chlorophylls, and interacts more tightly with the PSII core, inducing structural changes in the PSII antenna system, ultimately inhibiting the formation of PSII arrays and favouring plastoquinone diffusion. We suggest that dynamic Lhcb4 vs Lhcb8 expression allows for PSII acclimation to contrasting light conditions, offering the potential for engineering crops with improved light use efficiency. PubMed: 40715075DOI: 10.1038/s41467-025-62085-2 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3 Å) |
Structure validation
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