9LK4

Cryo-EM structure of Lhcb4.1-C2S2 PSII-LHCII supercomplex from Arabidopsis thaliana

9LK4 の概要

• エントリーDOI: 10.2210/pdb9lk4/pdb
• EMDBエントリー: 63167
• 分子名称: Photosystem II protein D1, Photosystem II reaction center protein J, Photosystem II reaction center protein K, ... (37 entities in total)
• 機能のキーワード: photosystem ii, light harvesting, photoacclimation, stress-induced antennae, cryo-em, photosynthesis
• 由来する生物種: Arabidopsis thaliana (thale cress); 詳細
• タンパク質・核酸の鎖数: 46
• 化学式量合計: 1182946.43

構造登録者

Zhou, Q.,Caferri, R.,Shan, J.Y.,Amelii, A.,Bassi, R.,Liu, Z.F. (登録日: 2025-01-16, 公開日: 2025-06-18, 最終更新日: 2025-08-13)

主引用文献

Caferri, R.,Zhou, Q.,Dall'Osto, L.,Amelii, A.,Shan, J.,Liu, Z.,Bassi, R.
A stress-induced paralog of Lhcb4 controls the photosystem II functional architecture in Arabidopsis thaliana.
Nat Commun, 16:6910-6910, 2025

PubMed Abstract: Photosystem II (PSII) is the pigment-protein complex catalysing light-induced water oxidation. In Arabidopsis thaliana, it includes three Lhcb4-6 proteins linking the core complex to peripheral trimeric antennae. While Lhcb5 and Lhcb6 are encoded by single genes, Lhcb4 is encoded by three isoforms: Lhcb4.1 and Lhcb4.2, constitutively expressed, and Lhcb4.3 (Lhcb8), which accumulates under prolonged abiotic stress. Lhcb8 substitutes for Lhcb4, preventing Lhcb6 accumulation and resulting in a smaller PSII with high quantum yield. Cryo-electron microscopy reveals that Lhcb8 has a shorter carboxy-terminal domain, lacks two chlorophylls, and interacts more tightly with the PSII core, inducing structural changes in the PSII antenna system, ultimately inhibiting the formation of PSII arrays and favouring plastoquinone diffusion. We suggest that dynamic Lhcb4 vs Lhcb8 expression allows for PSII acclimation to contrasting light conditions, offering the potential for engineering crops with improved light use efficiency.

PubMed: 40715075
DOI: 10.1038/s41467-025-62085-2

実験手法

ELECTRON MICROSCOPY (3.1 Å)