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データを開く
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基本情報
| 登録情報 | ![]() | ||||||||||||
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| タイトル | Cryo-EM structure of Lhcb4.1-C2S2 PSII-LHCII supercomplex from Arabidopsis thaliana | ||||||||||||
マップデータ | |||||||||||||
試料 |
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キーワード | Photosystem II / light harvesting / photoacclimation / stress-induced antennae / cryo-EM / PHOTOSYNTHESIS | ||||||||||||
| 機能・相同性 | 機能・相同性情報response to desiccation / chloroplast photosystem II / photoinhibition / photosynthesis, light harvesting in photosystem II / photosystem II antenna complex / response to red light / cellular response to abscisic acid stimulus / cellular response to water deprivation / nonphotochemical quenching / PSII associated light-harvesting complex II ...response to desiccation / chloroplast photosystem II / photoinhibition / photosynthesis, light harvesting in photosystem II / photosystem II antenna complex / response to red light / cellular response to abscisic acid stimulus / cellular response to water deprivation / nonphotochemical quenching / PSII associated light-harvesting complex II / chloroplast stromal thylakoid / response to low light intensity stimulus / thylakoid lumen / plastid thylakoid membrane / plastoglobule / regulation of stomatal closure / response to far red light / thylakoid membrane / response to high light intensity / chloroplast thylakoid / vacuolar transport / photosynthesis, light harvesting in photosystem I / photosystem II oxygen evolving complex / apoplast / photosystem II assembly / thylakoid / chloroplast thylakoid lumen / oxygen evolving activity / photosystem II stabilization / photosystem II reaction center / photosystem II / protein phosphatase regulator activity / response to fructose / chloroplast envelope / plant-type vacuole / photosynthetic electron transport chain / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosystem I / response to herbicide / photosystem II / chloroplast stroma / poly(U) RNA binding / plastid / photosynthetic electron transport in photosystem II / chlorophyll binding / phosphate ion binding / photosynthesis, light reaction / chloroplast thylakoid membrane / positive regulation of reactive oxygen species biosynthetic process / response to light stimulus / photosynthesis / response to cold / chloroplast / electron transfer activity / protein stabilization / iron ion binding / protein domain specific binding / mRNA binding / heme binding / mitochondrion / metal ion binding / nucleus / plasma membrane / cytosol 類似検索 - 分子機能 | ||||||||||||
| 生物種 | ![]() | ||||||||||||
| 手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.1 Å | ||||||||||||
データ登録者 | Zhou Q / Caferri R / Shan JY / Amelii A / Bassi R / Liu ZF | ||||||||||||
| 資金援助 | European Union, 中国, 3件
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引用 | ジャーナル: Nat Commun / 年: 2025タイトル: A stress-induced paralog of Lhcb4 controls the photosystem II functional architecture in Arabidopsis thaliana. 著者: Roberto Caferri / Qian Zhou / Luca Dall'Osto / Antonello Amelii / Jianyu Shan / Zhenfeng Liu / Roberto Bassi / ![]() 要旨: Photosystem II (PSII) is the pigment-protein complex catalysing light-induced water oxidation. In Arabidopsis thaliana, it includes three Lhcb4-6 proteins linking the core complex to peripheral ...Photosystem II (PSII) is the pigment-protein complex catalysing light-induced water oxidation. In Arabidopsis thaliana, it includes three Lhcb4-6 proteins linking the core complex to peripheral trimeric antennae. While Lhcb5 and Lhcb6 are encoded by single genes, Lhcb4 is encoded by three isoforms: Lhcb4.1 and Lhcb4.2, constitutively expressed, and Lhcb4.3 (Lhcb8), which accumulates under prolonged abiotic stress. Lhcb8 substitutes for Lhcb4, preventing Lhcb6 accumulation and resulting in a smaller PSII with high quantum yield. Cryo-electron microscopy reveals that Lhcb8 has a shorter carboxy-terminal domain, lacks two chlorophylls, and interacts more tightly with the PSII core, inducing structural changes in the PSII antenna system, ultimately inhibiting the formation of PSII arrays and favouring plastoquinone diffusion. We suggest that dynamic Lhcb4 vs Lhcb8 expression allows for PSII acclimation to contrasting light conditions, offering the potential for engineering crops with improved light use efficiency. | ||||||||||||
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構造の表示
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ダウンロードとリンク
-EMDBアーカイブ
| マップデータ | emd_63167.map.gz | 220.1 MB | EMDBマップデータ形式 | |
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| ヘッダ (付随情報) | emd-63167-v30.xml emd-63167.xml | 42.6 KB 42.6 KB | 表示 表示 | EMDBヘッダ |
| 画像 | emd_63167.png | 117.1 KB | ||
| Filedesc metadata | emd-63167.cif.gz | 10.5 KB | ||
| アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-63167 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-63167 | HTTPS FTP |
-関連構造データ
| 関連構造データ | ![]() 9lk4MC ![]() 9lk5C M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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| 類似構造データ | 類似検索 - 機能・相同性 F&H 検索 |
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リンク
| EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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| 「今月の分子」の関連する項目 |
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マップ
| ファイル | ダウンロード / ファイル: emd_63167.map.gz / 形式: CCP4 / 大きさ: 244.1 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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| 投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
| ボクセルのサイズ | X=Y=Z: 1.06 Å | ||||||||||||||||||||||||||||||||||||
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| 対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
| 詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : Cryo-EM structure of the Lhcb8-C2S2 PSII-LHCII supercomplex from ...
+超分子 #1: Cryo-EM structure of the Lhcb8-C2S2 PSII-LHCII supercomplex from ...
+分子 #1: Photosystem II protein D1
+分子 #2: Photosystem II CP47 reaction center protein
+分子 #3: Photosystem II CP43 reaction center protein
+分子 #4: Photosystem II D2 protein
+分子 #5: Cytochrome b559 subunit alpha
+分子 #6: Cytochrome b559 subunit beta
+分子 #7: Chlorophyll a-b binding protein 2, chloroplastic
+分子 #8: Photosystem II reaction center protein H
+分子 #9: Photosystem II reaction center protein I
+分子 #10: Photosystem II reaction center protein J
+分子 #11: Photosystem II reaction center protein K
+分子 #12: Photosystem II reaction center protein L
+分子 #13: Photosystem II reaction center protein M
+分子 #14: Oxygen-evolving enhancer protein 1-1, chloroplastic
+分子 #15: Chlorophyll a-b binding protein CP29.1, chloroplastic
+分子 #16: Photosystem II reaction center protein T
+分子 #17: Photosystem II 5 kDa protein, chloroplastic
+分子 #18: Photosystem II reaction center W protein, chloroplastic
+分子 #19: (thale cress) hypothetical protein
+分子 #20: Chlorophyll a-b binding protein 2.2, chloroplastic
+分子 #21: Photosystem II reaction center protein Z
+分子 #22: Chlorophyll a-b binding protein CP26, chloroplastic
+分子 #23: CHLOROPHYLL A
+分子 #24: PHEOPHYTIN A
+分子 #25: BETA-CAROTENE
+分子 #26: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
+分子 #27: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+分子 #28: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
+分子 #29: FE (II) ION
+分子 #30: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+分子 #31: BICARBONATE ION
+分子 #32: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,...
+分子 #33: PROTOPORPHYRIN IX CONTAINING FE
+分子 #34: CHLOROPHYLL B
+分子 #35: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
+分子 #36: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY...
+分子 #37: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BE...
-実験情報
-構造解析
| 手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
| 試料の集合状態 | particle |
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試料調製
| 緩衝液 | pH: 7.5 |
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| 凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
| 顕微鏡 | TFS KRIOS |
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| 撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 60.0 e/Å2 |
| 電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
| 電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.0 µm / 最小 デフォーカス(公称値): 1.0 µm |
| 実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
ムービー
コントローラー
万見について




キーワード
データ登録者
中国, 3件
引用










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Y (Row.)
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解析
FIELD EMISSION GUN

