9LD8

Crystal structure of LSSmOrange under room temperature at pH 8.0

Summary for 9LD8

• Entry DOI: 10.2210/pdb9ld8/pdb
• Descriptor: mOrange (2 entities in total)
• Functional Keywords: fluorescent protein, neutral ph, sfx, room temperature.
• Biological source: Discosoma sp.
• Total number of polymer chains: 1
• Total formula weight: 27805.41

Authors

Luo, F.,De Zitter, E.,Nango, E.,Mizuno, H. (deposition date: 2025-01-05, release date: 2026-04-22, Last modification date: 2026-06-17)

Primary citation

Seol, H.S.,Luo, F.,De Zitter, E.,Nuemket, N.,Fron, E.,McFarlane, N.R.,De Vrieze, L.,Civic, J.,Postelmans, M.,Van Bel, S.,Owada, S.,Tono, K.,Tanaka, T.,Arima, T.,Noguchi, H.,Bui, T.Y.H.,Tanaka, R.,Hasegawa, K.,Hirata, K.,Im, D.,Araya, T.,Kimura, T.,Van Meervelt, L.,Weik, M.,Harvey, J.N.,Colletier, J.P.,Iwata, S.,Nango, E.,Mizuno, H.
Decarboxylation via a Higher Electronic Excited State Drives LSSmOrange Photoconversion.
ACS Phys Chem Au, 6:534-551, 2026

PubMed Abstract: LSSmOrange is a fluorescent protein that exhibits slow photoconversion and is used as an imaging tool to highlight specific subpopulations of molecules. While photoconversion in LSSmOrange is known to involve Kolbe decarboxylation of the E215 side chain, its structural dynamics remain unexplored. We addressed the excited state dynamics of LSSmOrange by using serial femtosecond crystallography (SFX) with an X-ray free electron laser. SFX enabled us to determine the crystal structure of unconverted LSSmOrange without detectable X-ray damage, facilitating a femtosecond optical pumpX-ray probe experiment to track time-resolved structural changes. A decrease in electron density at E215 was observed 250 ps after strong pump laser illumination (mean fluence of 0.31 J/cm), consistent with photoconversion by decarboxylation. Extrapolated structures suggested the appearance of an alternative E215 conformation in addition to illumination-induced decarboxylation. Since the photoinduced decarboxylation occurred despite the extremely low single-photon photoconversion quantum yield of LSSmOrange (8.5 × 10), we hypothesized that the photoconversion proceeds via a multiphoton process. Power titration using transient absorption spectroscopy revealed that photoconversion was linked to optical nonlinearity under a high pump laser energy. We propose that LSSmOrange photoconversion involves a higher-lying electronic excited state triggered by multiphoton absorption, as supported by quantum-chemical calculations. Photoconversion via a multiphoton process enables spatially confined highlighting on a microscope.

PubMed: 42221925
DOI: 10.1021/acsphyschemau.6c00009

Experimental method

X-RAY DIFFRACTION (1.7 Å)