9KOY

Crystal structure of Oryza sativa HPPD complexed with iptriazopyrid

これはPDB形式変換不可エントリーです。

9KOY の概要

• エントリーDOI: 10.2210/pdb9koy/pdb
• 関連するPDBエントリー: 9KOZ 9KP0
• 分子名称: 4-hydroxyphenylpyruvate dioxygenase, COBALT (II) ION, iptriazopyrid, ... (5 entities in total)
• 機能のキーワード: inhibitor, complex, oxidoreductase, oxidoreducatase-inhibitor complex, oxidoreducatase/inhibitor
• 由来する生物種: Oryza sativa
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 95212.54

構造登録者

Nishio, T.,Nishijima, N.,Kubota, T.,Furuhata, Y.,Kato, Y. (登録日: 2024-11-22, 公開日: 2025-06-25, 最終更新日: 2025-07-02)

主引用文献

Nishio, T.,Nishijima, N.,Kubota, T.,Furuhata, Y.,Nanao, Y.,Permana, H.,Furuhashi, T.,Kato, Y.
Mechanism of Herbicidal Action and Rice Selectivity of Iptriazopyrid: A Novel Azole Carboxamide-Based Inhibitor of 4-Hydroxyphenylpyruvate Dioxygenase.
J.Agric.Food Chem., 73:15950-15958, 2025

PubMed Abstract: 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors are widely used as herbicides. However, the emergence of herbicide-resistant weeds necessitates the development of new herbicides with more diverse chemical structures. Therefore, we evaluated the herbicidal and HPPD inhibitory activities of iptriazopyrid, a novel azole carboxamide compound. Phytotoxic tests on demonstrated that iptriazopyrid caused chlorosis at a concentration approximately 10 times lower than that required for the common commercial HPPD inhibitor mesotrione. Furthermore, iptriazopyrid showed strong selectivity for over the weed in the greenhouse-scale experiment. X-ray crystallography and inhibitory assays revealed that iptriazopyrid was bound to the enzyme active pocket of HPPD and worked as a slow-binding inhibitor. These findings indicate that this inhibitor has potent herbicidal activity with a chemical structure different from those of existing HPPD inhibitors. Thus, it has potential applications as a novel scaffold in herbicide development.

PubMed: 40495343
DOI: 10.1021/acs.jafc.4c11831

実験手法

X-RAY DIFFRACTION (2.9 Å)