9KKC

Neutron structure of Ferredoxin-NADP+ reductase from maize root -Oxidized form

9KKC の概要

• エントリーDOI: 10.2210/pdb9kkc/pdb
• 分子名称: Ferredoxin--NADP reductase, chloroplastic, FLAVIN-ADENINE DINUCLEOTIDE, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (4 entities in total)
• 機能のキーワード: electron transfer, flavoprotein, oxidoreductase
• 由来する生物種: Zea mays
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35584.98

構造登録者

Uenaka, M.,Ohnishi, Y.,Tanaka, H.,Kurisu, G. (登録日: 2024-11-13, 公開日: 2025-01-29, 最終更新日: 2025-03-12)

主引用文献

Uenaka, M.,Ohnishi, Y.,Ise, A.,Yu, J.,Yano, N.,Kusaka, K.,Tanaka, H.,Kurisu, G.
Redox-dependent hydrogen-bond network rearrangement of ferredoxin-NADP + reductase revealed by high-resolution X-ray and neutron crystallography.
Acta Crystallogr.,Sect.F, 81:73-84, 2025

PubMed Abstract: High-resolution X-ray and neutron crystallography were employed to elucidate redox-dependent structural changes in ferredoxin-NADP reductase (FNR) from maize. This study focused on the rearrangement of hydrogen-bond networks upon FAD reduction. The X-ray structures of wild-type FNR in oxidized and reduced states were refined to 1.15 and 1.10 Å resolution, respectively, revealing no large structural changes in the main-chain backbones. Neutron crystallography provided complementary insights, confirming protonation at N1 and N5 of the isoalloxazine ring and visualizing hydrogen bonds that were undetectable by X-ray analysis. These findings illuminate the dynamic reorganization of water-mediated hydrogen-bond networks during redox transitions, which may underpin the redox-dependent modulation of partner binding by FNR. This integrated structural approach highlights the synergistic use of X-ray and neutron crystallography in studying redox-active proteins.

PubMed: 39913263
DOI: 10.1107/S2053230X25000524

実験手法

NEUTRON DIFFRACTION (1.8 Å)