9KHA

Crystal structure of maltodextrin-binding protein SPs0871 from Streptococcus pyogenes at 2.20 A

9KHA の概要

• エントリーDOI: 10.2210/pdb9kha/pdb
• 分子名称: Extracellular solute-binding protein (2 entities in total)
• 機能のキーワード: streptococcus pyogenes; virtual screening; biophysical-based screening; small compounds, sugar binding protein
• 由来する生物種: Streptococcus pyogenes M1 GAS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 83995.61

構造登録者

Caaveiro, J.M.M.,Yamawaki, T.,Tsumoto, K. (登録日: 2024-11-09, 公開日: 2025-06-25)

主引用文献

Yamawaki, T.,Nakakido, M.,Nagatoishi, S.,Caaveiro, J.M.M.,Kuroda, D.,Aikawa, C.,Nakagawa, I.,Tsumoto, K.
Development of a small compound that regulates the function of a maltodextrin-binding protein of Streptococcus pyogenes by multifaceted screenings.
Sci Rep, 15:19341-19341, 2025

PubMed Abstract: Group A Streptococcus (GAS) are gram-positive bacteria that cause various symptoms. The treatment of GAS infections currently relies on antibiotics, but new treatment options are needed due to the spread of antibiotic resistance. To develop novel treatment methods that circumvent the generation of antibiotic resistance, we used virtual screening followed by several biophysical-based screening methods to identify antibacterial compounds that target SPs0871, which is a maltodextrin-binding protein that is involved in carbohydrate catabolism in GAS. We narrowed down the list of compounds in the library via multi-step screening and finally isolated a compound that bacteriostatically inhibited the growth of GAS. Together with our previous study showing that an anti-SPs0871 variable heavy domain of heavy chain antibody, which completely blocked ligand binding, did not suppress bacterial growth, our results provide guidelines for designing an antistreptococcal therapeutic.

PubMed: 40456803
DOI: 10.1038/s41598-025-02175-9

実験手法

X-RAY DIFFRACTION (2.2 Å)