9KBD

Cryo-EM structure of the CUL1-RBX1-SKP1-FBXO3 SCF ubiquition ligase complex

9KBD の概要

• エントリーDOI: 10.2210/pdb9kbd/pdb
• EMDBエントリー: 62222
• 分子名称: S-phase kinase-associated protein 1, F-box only protein 3, E3 ubiquitin-protein ligase RBX1, ... (4 entities in total)
• 機能のキーワード: ubiquitination e3 ligase, cryo-em, protein binding, ligase
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 171075.89

構造登録者

Wei, J.,Xu, C. (登録日: 2024-10-30, 公開日: 2025-02-19, 最終更新日: 2025-07-23)

主引用文献

Wei, J.,Xu, C.
Structural Insight Into the SKP1-CUL1-FBXO3-RBX1 Complex.
Proteins, 93:1290-1294, 2025

PubMed Abstract: The cryo-EM structure of human SCF, which consists of CUL1, RBX1, SKP1 and FBXO3 was solved at a nominal resolution of 3.70 Å. Although a previous study reported the crystal structure of the FBXO3 ApaG domain, how FBXO3 is incorporated into the SCF complex remains elusive. In the cryo-EM structure of SCF, the F-box domain of FBXO3 primarily associates with SKP1 via extensive hydrophobic interactions and interacts with the N-terminal region of CUL1 via hydrophobic interactions. The weak cryo-EM map of the RBX1 globular region is close to the FBXO3 ApaG domain, suggesting that unmodified SCF exhibits a closed conformation and that CUL1 neddylation is likely required to achieve high E3 activity. The structural study provides insight into the assembly of SCF and its activation mediated by CUL1 neddylation.

PubMed: 39921442
DOI: 10.1002/prot.26809

実験手法

ELECTRON MICROSCOPY (3.7 Å)