9K42

Cryo-EM structure of Arabidopsis thaliana H2A-nucleosome with 147bp Widom 601 DNA (C2 symmetry)

9K42 の概要

• エントリーDOI: 10.2210/pdb9k42/pdb
• EMDBエントリー: 62039 62040
• 分子名称: Histone H3.1, Histone H4, Histone H2A.6, ... (6 entities in total)
• 機能のキーワード: nucleosome, histone, h2a, arabidopsis, nuclear protein/dna, nuclear protein-dna complex
• 由来する生物種: Arabidopsis thaliana (thale cress); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 204464.96

構造登録者

Wang, Y.,Dong, A. (登録日: 2024-10-21, 公開日: 2025-05-14, 最終更新日: 2025-05-28)

主引用文献

Wang, Y.,Wu, J.,Yang, S.,Li, X.,Wang, J.,Lv, Q.,Zhu, X.,Lu, G.,Zhang, J.,Shen, W.H.,Liu, B.,Lin, J.,Dong, A.
Structural and functional interrelationships of histone H2A with its variants H2A.Z and H2A.W in Arabidopsis.
Structure, 2025

PubMed Abstract: Multiple histone H2A variants are known in eukaryotes. However, the functional relationship between H2A and its variants in plants remains largely obscure. Using CRISPR-Cas9 editing, we generated a mutant lacking four H2A isoforms in Arabidopsis and analyzed the functional and structural relationships between H2A, H2A.Z, and H2A.W. RNA sequencing and phenotype analyses revealed mild changes in gene transcription and plant development in mutants lacking H2A, H2A.Z, or H2A.W compared with the wild-type plants. Chromatin immunoprecipitation sequencing analysis showed that H2A can substitute for both H2A.Z and H2A.W across the genome, including in euchromatin and heterochromatin regions. However, H2A.Z replaced both H2A and H2A.W primarily within the euchromatin regions. By using DNA and histones from Arabidopsis, we constructed nucleosomes containing H2A, H2A.Z, or H2A.W and resolved their cryogenic electron microscopy (cryo-EM) structures at near-atomic resolution. Collectively, the results reveal the structural similarity and functional redundancy of H2A and its variants in Arabidopsis.

PubMed: 40359943
DOI: 10.1016/j.str.2025.04.015

実験手法

ELECTRON MICROSCOPY (3.14 Å)