9K2C

Structure of ClpP from Staphylococcus aureus in complex with ZY1

これはPDB形式変換不可エントリーです。

9K2C の概要

• エントリーDOI: 10.2210/pdb9k2c/pdb
• 分子名称: ATP-dependent Clp protease proteolytic subunit, (6~{S},9~{a}~{S})-6-(1~{H}-imidazol-5-ylmethyl)-8-(naphthalen-1-ylmethyl)-4,7-bis(oxidanylidene)-~{N}-(phenylmethyl)-3,6,9,9~{a}-tetrahydro-2~{H}-pyrazino[1,2-a]pyrimidine-1-carboxamide, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: clpp, staphylococcus aureus, activator, protease, hydrolase, antibiotic
• 由来する生物種: Staphylococcus aureus subsp. aureus Mu3
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 325936.86

構造登録者

Li, J.H.,Wu, W.,Zhang, T.,Yang, C.-G. (登録日: 2024-10-17, 公開日: 2025-02-19)

主引用文献

Zhang, T.,Wu, W.,Zhao, Y.,Ding, Z.,Wei, B.,Yang, T.,Li, J.,Wang, P.,Lan, L.,Gan, J.,Yang, C.G.
Structure-Guided Development of ClpP Agonists with Potent Therapeutic Activities against Staphylococcus aureus Infection.
J.Med.Chem., 68:1810-1823, 2025

PubMed Abstract: Peritonitis caused by poses a severe threat to patients with end-stage renal failure. Treating multidrug-resistant infections requires the use of antibiotics with diverse mechanisms of action. Caseinolytic protease P (ClpP) is a promising antibacterial target; however, selective activation of (ClpP) over human ClpP (ClpP) remains challenging. We previously identified as a selective ClpP agonist, but its potency was suboptimal. Herein, we develop analogs through a structure-guided approach and examine their structure-activity relationships. Notably, demonstrates improved activation of ClpP and superior binding affinity. Interestingly, while facilitates the enzymatic hydrolysis of ClpP and ClpP , it does not target ClpP in cellular environments. Furthermore, effectively inhibits the growth of multidrug-resistant strains and shows excellent therapeutic efficacy in a murine model of peritonitis. These findings highlight as a promising ClpP agonist for combating staphylococcal infections.

PubMed: 39760203
DOI: 10.1021/acs.jmedchem.4c02562

実験手法

X-RAY DIFFRACTION (1.98 Å)