9JUS

Structure of villin bound to an actin trimer

9JUS の概要

• エントリーDOI: 10.2210/pdb9jus/pdb
• 分子名称: Actin, alpha skeletal muscle, villin, ADENOSINE-5'-DIPHOSPHATE, ... (8 entities in total)
• 機能のキーワード: villin actin nucleus, structural protein
• 由来する生物種: Gallus gallus (chicken); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 442879.25

構造登録者

Robinson, R.C. (登録日: 2024-10-08, 公開日: 2025-10-08, 最終更新日: 2025-12-17)

主引用文献

Robinson, R.C.,Chongrungreang, T.,Ponlachantra, K.,Boonyarit, B.,Dilly, G.F.,Li, Y.I.,Girguis, P.R.,Copley, R.R.,Claridge-Chang, A.
The structure of an actin nucleus stabilized by villin.
Sci Adv, 11:eadw6915-eadw6915, 2025

PubMed Abstract: Villin is an actin filament nucleating, severing, capping and bundling protein; however, the structural basis for villin's functions and the characteristics of the actin polymerization nucleus remain poorly understood. Here, we present the structure of vent-worm villin bound to a trimeric actin nucleus. Villin wraps around and caps the barbed end of the actin trimer. Its headpiece domain interacts at the junction of two laterally associated actin protomers, leaving the pointed-end subunits open for elongation. Within the actin trimer, the two longitudinally associated subunits adopt barbed and pointed-end subunit conformations, while the lateral protomer exhibits a monomeric conformation. This provides the first view of an actin-filament nucleus, revealing that the transition into the filamentous form is stimulated and stabilized by the interactions with the pointed-end subunits. Our results also illuminate mechanisms of actin-filament dynamics and villin capping and severing, suggesting that F-to-G actin conformational transitions facilitate the later process.

PubMed: 41337577
DOI: 10.1126/sciadv.adw6915

実験手法

X-RAY DIFFRACTION (2.7 Å)