9JKP

Crystal structure of the Staphylococcus aureus GTPase Era in complex with GDP

9JKP の概要

• エントリーDOI: 10.2210/pdb9jkp/pdb
• 分子名称: GTPase Era, GUANOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: ribosome, maturation, gtpase, rna binding protein
• 由来する生物種: Staphylococcus aureus (strain NCTC 8325 / PS 47)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34847.25

構造登録者

Klochkova, E.,Biktimirov, A.,Islamov, D.,Belousov, A.,Yusupov, M.,Usachev, K. (登録日: 2024-09-16, 公開日: 2024-12-18)

主引用文献

Klochkova, E.,Biktimirov, A.,Islamov, D.,Belousov, A.,Validov, S.,Yusupov, M.,Usachev, K.
Crystal structure of the GDP-bound GTPase Era from Staphylococcus aureus.
Biochem.Biophys.Res.Commun., 735:150852-150852, 2024

PubMed Abstract: GTPase Era from Staphylococcus aureus belongs to the TRAFAC superfamily of the TrmE-Era-EngA-EngB-Septin-like GTPases class and plays a significant role in the vital activity of this pathogenic microorganism as a maturation factor of the 30S ribosome subunit. However, the functions of this protein are not fully understood, making it a promising object for further study. Here, the 2.76 Å resolution crystal structure of Staphylococcus aureus Era in complex with GDP is presented. Structural comparison with other GTP-bound and GDP-bound homologous proteins, GTPase domain and the KH domain revealed a mutual orientation in S. aureus which has not been described before. The GDP-bound Era structure presented here will facilitate efforts to elucidate its interactions with its regulators and lay the foundation for a structure-based search for specific inhibitors.

PubMed: 39432921
DOI: 10.1016/j.bbrc.2024.150852

実験手法

X-RAY DIFFRACTION (2.76 Å)