9JK7

Human VANGL2 hexamer

Summary for 9JK7

• Entry DOI: 10.2210/pdb9jk7/pdb
• EMDB information: 61546
• Descriptor: Vang-like protein 2 (1 entity in total)
• Functional Keywords: vangl, planar cell polarity, pcp, membrane protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 6
• Total formula weight: 362244.44

Authors

Song, Y.,Zhang, Z.,Jian, S.,Zheng, P. (deposition date: 2024-09-15, release date: 2024-12-25, Last modification date: 2025-01-22)

Primary citation

Song, Y.,Jian, S.,Teng, J.,Zheng, P.,Zhang, Z.
Structural basis of human VANGL-PRICKLE interaction.
Nat Commun, 16:132-132, 2025

PubMed Abstract: Planar cell polarity (PCP) is an evolutionarily conserved process for development and morphogenesis in metazoans. The well-organized polarity pattern in cells is established by the asymmetric distribution of two core protein complexes on opposite sides of the cell membrane. The Van Gogh-like (VANGL)-PRICKLE (PK) pair is one of these two key regulators; however, their structural information and detailed functions have been unclear. Here, we present five cryo-electron microscopy structures of human VANGL1, VANGL2, and their complexes with PK1 at resolutions of 2.2-3.0 Å. Through biochemical and cell imaging experiments, we decipher the molecular details of the VANGL-PK interaction. Furthermore, we reveal that PK1 can target VANGL-containing intracellular vesicles to the peripheral cell membrane. These findings provide a solid foundation to understand the explicit interaction between VANGL and PK while opening new avenues for subsequent studies of the PCP pathway.

PubMed: 39753555
DOI: 10.1038/s41467-024-55396-3

Experimental method

ELECTRON MICROSCOPY (2.9 Å)