9J8V

TSWV L protein in complex with ribavirin 5-triphosphate

9J8V の概要

• エントリーDOI: 10.2210/pdb9j8v/pdb
• EMDBエントリー: 61241
• 分子名称: RNA-directed RNA polymerase L, RIBAVIRIN TRIPHOSPHATE (2 entities in total)
• 機能のキーワード: tswv, l protein, ribavirin 5-triphosphate, viral protein
• 由来する生物種: Orthotospovirus tomatomaculae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 204149.02

構造登録者

Cao, L.,Wang, X. (登録日: 2024-08-21, 公開日: 2025-04-16, 最終更新日: 2025-07-23)

主引用文献

Li, J.,Cao, L.,Zhao, Y.,Shen, J.,Wang, L.,Feng, M.,Zhu, M.,Ye, Y.,Kormelink, R.,Tao, X.,Wang, X.
Structural basis for the activation of plant bunyavirus replication machinery and its dual-targeted inhibition by ribavirin.
Nat.Plants, 11:518-530, 2025

PubMed Abstract: Despite the discovery of plant viruses as a new class of pathogens over a century ago, the structure of plant virus replication machinery and antiviral pesticide remains lacking. Here we report five cryogenic electron microscopy structures of a ~330-kDa RNA-dependent RNA polymerase (RdRp) from a devastating plant bunyavirus, tomato spotted wilt orthotospovirus (TSWV), including the apo, viral-RNA-bound, base analogue ribavirin-bound and ribavirin-triphosphate-bound states. They reveal that a flexible loop of RdRp's motif F functions as 'sensor' to perceive viral RNA and further acts as an 'adaptor' to promote the formation of a complete catalytic centre. A ten-base RNA 'hook' structure is sufficient to trigger major conformational changes and activate RdRp. Chemical screening showed that ribavirin is effective against TSWV, and structural data revealed that ribavirin disrupts both hook-binding and catalytic core formation, locking polymerase in its inactive state. This work provides structural insights into the mechanisms of plant bunyavirus RdRp activation and its dual-targeted site inhibition, facilitating the development of pesticides against plant viruses.

PubMed: 40044941
DOI: 10.1038/s41477-025-01940-y

実験手法

ELECTRON MICROSCOPY (3.1 Å)