9J6I

Crystal structure of the ABA receptor PYL1 in complex with DBSA compound

Summary for 9J6I

• Entry DOI: 10.2210/pdb9j6i/pdb
• Descriptor: Abscisic acid receptor PYL1, 5-bromanyl-N2-[(4-bromophenyl)-bis(oxidanyl)-$l^4-sulfanyl]-N1,N1,N3,N3-tetrakis(oxidanyl)benzene-1,2,3-triamine (3 entities in total)
• Functional Keywords: aba receptor, pyl1, lipid binding protein
• Biological source: Arabidopsis thaliana (thale cress)
• Total number of polymer chains: 2
• Total formula weight: 41040.51

Authors

Yan, J. (deposition date: 2024-08-16, release date: 2024-09-04, Last modification date: 2024-10-16)

Primary citation

Wang, Z.Z.,Cao, M.J.,Yan, J.,Dong, J.,Chen, M.X.,Yang, J.F.,Li, J.H.,Ying, R.N.,Gao, Y.Y.,Li, L.,Leng, Y.N.,Tian, Y.,Hewage, K.A.H.,Pei, R.J.,Huang, Z.Y.,Yin, P.,Zhu, J.K.,Hao, G.F.,Yang, G.F.
Stabilization of dimeric PYR/PYL/RCAR family members relieves abscisic acid-induced inhibition of seed germination.
Nat Commun, 15:8077-8077, 2024

PubMed Abstract: Abscisic acid (ABA) is the primary preventing factor of seed germination, which is crucial to plant survival and propagation. ABA-induced seed germination inhibition is mainly mediated by the dimeric PYR/PYL/RCAR (PYLs) family members. However, little is known about the relevance between dimeric stability of PYLs and seed germination. Here, we reveal that stabilization of PYL dimer can relieve ABA-induced inhibition of seed germination using chemical genetic approaches. Di-nitrobensulfamide (DBSA), a computationally designed chemical probe, yields around ten-fold improvement in receptor affinity relative to ABA. DBSA reverses ABA-induced inhibition of seed germination mainly through dimeric receptors and recovers the expression of ABA-responsive genes. DBSA maintains PYR1 in dimeric state during protein oligomeric state experiment. X-ray crystallography shows that DBSA targets a pocket in PYL dimer interface and may stabilize PYL dimer by forming hydrogen networks. Our results illustrate the potential of PYL dimer stabilization in preventing ABA-induced seed germination inhibition.

PubMed: 39277642
DOI: 10.1038/s41467-024-52426-y

Experimental method

X-RAY DIFFRACTION (2.3 Å)