9IQ8

Ankyrin-like protein, AnkB

Summary for 9IQ8

• Entry DOI: 10.2210/pdb9iq8/pdb
• Descriptor: Ankyrin-like protein AnkB (2 entities in total)
• Functional Keywords: ankb, ankyrin repeat domain-containing protein, signal transduction mechanisms, signaling protein
• Biological source: Acinetobacter baumannii
• Total number of polymer chains: 4
• Total formula weight: 70352.44

Authors

Sung, J.H.,Park, H.H. (deposition date: 2024-07-12, release date: 2025-07-16)

Primary citation

Sung, J.H.,Lee, S.Y.,Lee, C.S.,Lee, J.H.,Park, H.H.
Structural analysis of a bacterial ankyrin-like protein secreted by Acinetobacter baumannii.
Biochem.Biophys.Res.Commun., 733:150573-150573, 2024

PubMed Abstract: In bacteria, the ankyrin-like protein AnkB helps overcome stress by regulating catalase activity when expressed under stressful conditions. As the structural properties of AnkB are largely unexplored, our understanding of various AnkB-mediated functions in bacteria remains limited. In the present study, we describe the structure of AnkB from Acinetobacter baumannii, hereafter referred to as "AbAnkB," which has a unique tertiary configuration compared with that of other ankyrin domain-containing proteins. Structural analysis revealed that AbAnkB has a relatively long loop between AKR3 and AKR4 and an oppositely positioned α helix. Based on amino acid conservation and protein surface analyses, we identified a hydrophobic patch that might be critical for the function of AbAnkB. To the best of our knowledge, our study is the first to report the structure of a bacterial AnkB protein; our findings will markedly enhance our understanding of its functions in bacteria.

PubMed: 39208644
DOI: 10.1016/j.bbrc.2024.150573

Experimental method

X-RAY DIFFRACTION (2.42 Å)