9ION

Cryo-EM structure of cUA bound CapE filament

これはPDB形式変換不可エントリーです。

9ION の概要

• エントリーDOI: 10.2210/pdb9ion/pdb
• EMDBエントリー: 60745
• 分子名称: cUMP-AMP-activated phospholipase, 3'3'-cUMP-AMP (2 entities in total)
• 機能のキーワード: cape, patatin-like phospholipase protein, lipid binding protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 144169.24

構造登録者

Gao, A.,Wang, J.G. (登録日: 2024-07-09, 公開日: 2025-05-21, 最終更新日: 2025-07-23)

主引用文献

Wang, J.,Li, Z.,Lang, H.,Fu, W.,Gao, Y.,Yin, S.,Sun, P.,Li, Z.,Huang, J.,Liu, S.,Zhu, Y.,Sun, F.,Li, D.,Gao, P.
Cyclic-dinucleotide-induced filamentous assembly of phospholipases governs broad CBASS immunity.
Cell, 188:3744-3756.e16, 2025

PubMed Abstract: Cyclic-oligonucleotide-based antiphage signaling systems (CBASS), a widespread antiviral bacterial immune system homologous to the mammalian cGAS-STING pathway, synthesizes cyclic nucleotide signals and triggers effector proteins to induce cell death and prevent viral propagation. Among various CBASS effectors, phospholipase effectors are the first to be discovered and are one of the most widespread families that sense cyclic dinucleotides to degrade cell membrane phospholipids. Here, we report that CBASS phospholipases assemble from a dimeric inactive state into active higher-order filamentous oligomers upon sensing cyclic dinucleotides. Using a combined approach of cryo-electron microscopy and X-ray crystallography, we have determined the structures of CBASS phospholipase in the inactive dimeric state, the cyclic-dinucleotide-bound active higher-order state, and the substrate-analog-bound catalytic mimicry state, thereby visualizing the complete conformational reorganization process. Complemented by functional assays of intermolecular binding, phospholipase enzymatic activity, in vitro membrane disruption, and in vivo antiphage efficiency, our work elucidates the mechanisms of assembly and activation of CBASS phospholipases.

PubMed: 40345202
DOI: 10.1016/j.cell.2025.04.022

実験手法

ELECTRON MICROSCOPY (3.27 Å)