9INV

Crystal structure of DAPK1 in complex with isoliquiritigenin

9INV の概要

• エントリーDOI: 10.2210/pdb9inv/pdb
• 分子名称: Death-associated protein kinase 1, 2',4,4'-TRIHYDROXYCHALCONE, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: dapk1, kinase, inhibitor, isoliquiritigenin, transferase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34148.72

構造登録者

Yokoyama, T. (登録日: 2024-07-08, 公開日: 2024-10-09)

主引用文献

Yokoyama, T.,Hisatomi, K.,Oshima, S.,Tanaka, I.,Okada, T.,Toyooka, N.
Discovery and optimization of isoliquiritigenin as a death-associated protein kinase 1 inhibitor.
Eur.J.Med.Chem., 279:116836-116836, 2024

PubMed Abstract: Death-associated protein kinase 1 (DAPK1) is a phosphotransferase in the serine/threonine kinase family. Inhibiting DAPK1 is expected to be beneficial in treating Alzheimer's disease and protecting neuronal cells during cerebral ischemia. In this study, we demonstrated that the natural chalcone isoliquiritigenin inhibits DAPK1 in an ATP-competitive manner, and we synthesized halogen derivatives to amplify the inhibitory effect. Among the compounds tested, the chlorine, bromine, and iodine derivatives exhibited high DAPK1 inhibitory activity and binding affinity. Crystal structure analysis revealed that this improvement is attributable to the halogen atoms fitting well into the hydrophobic pocket formed by I77, L93, and I160. In particular, the chlorine derivative showed a significant enthalpic contribution to the interaction with DAPK1, suggesting its potential as a primary compound for new DAPK1 inhibitors.

PubMed: 39243455
DOI: 10.1016/j.ejmech.2024.116836

実験手法

X-RAY DIFFRACTION (1.61 Å)