9ILS

The GmvT toxin in complex with the C-terminal fragment of its antitoxin

9ILS の概要

• エントリーDOI: 10.2210/pdb9ils/pdb
• 分子名称: N-acetyltransferase, DUF1778 domain-containing protein, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: toxin-antitoxin system, gnat, acetylation, accoa, gmvat, toxin
• 由来する生物種: Shigella sonnei; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 23939.33

構造登録者

Xie, W.,Chen, R.,Zhao, H. (登録日: 2024-07-01, 公開日: 2025-03-05, 最終更新日: 2025-05-28)

主引用文献

Chen, R.,Zhao, H.,Zhou, J.,Liu, A.,Guo, Y.,Wu, K.,Xiang, Y.,Lei, J.,Jiang, S.,Xie, W.
Structural insights into the Shigella flexneri GmvAT toxin-antitoxin system.
Febs Lett., 599:1246-1259, 2025

PubMed Abstract: Toxin-antitoxin (TA) systems are common bicistronic gene elements in bacteria and are critical for stress responses. The toxin members of the GNAT/RHH TA family can acetylate certain aminoacylated tRNA molecules and inhibit global protein translation. One member named GmvT is important for virulence plasmid maintenance in Shigella flexneri, but the underlying mechanism remains poorly understood. Here, we report the cocrystal structures of GmvT in two forms. The binding of the antitoxin mainly relies on the backbone of the toxin while the cofactor is free of contacts with the antitoxin, supported by follow-up in vitro and in vivo studies. Our study provides insight into the protein-protein/protein-ligand interactions of the GmvAT pair and the structural basis for molecular recognition.

PubMed: 39973444
DOI: 10.1002/1873-3468.70015

実験手法

X-RAY DIFFRACTION (2.75 Å)