9IID

Crystal structure of PgAfp

9IID の概要

• エントリーDOI: 10.2210/pdb9iid/pdb
• 分子名称: Antifungal protein B (2 entities in total)
• 機能のキーワード: pgafp, antimicrobial protein
• 由来する生物種: Penicillium chrysogenum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6670.56

構造登録者

Wang, Y. (登録日: 2024-06-20, 公開日: 2024-07-10, 最終更新日: 2025-05-21)

主引用文献

Wang, Y.,Wang, S.,Chen, Y.,Xie, C.,Xu, H.,Lin, Y.,Lin, R.,Zeng, W.,Chen, X.,Nie, X.,Wang, S.
The role of Npt1 in regulating antifungal protein activity in filamentous fungi.
Nat Commun, 16:2850-2850, 2025

PubMed Abstract: Pathogenic filamentous fungi pose a significant threat to global food security and human health. The limitations of available antifungal agents, including resistance and toxicity, highlight the need for developing innovative antifungal strategies. Antifungal proteins (AFPs) are a class of secreted small proteins that exhibit potent antifungal activity against filamentous fungi, yet the underlying mechanism remains partially understood. In this study, we investigate the molecular and cellular effects of two AFPs, PgAFP and AfAFP, on Aspergillus flavus, a representative filamentous fungus. These AFPs affect various fungal phenotypes and exert an intracellular effect by interacting with Ntp1, a fungi exclusive protein modulating diverse fungal traits. We find that Ntp1 amino acids 417-588 are critical for AFP binding and play a role in regulating growth, development, sporulation, sclerotia formation, toxin synthesis, and pathogenicity. Results generated from this study will help to control pathogenic fungi.

PubMed: 40122888
DOI: 10.1038/s41467-025-58230-6

実験手法

X-RAY DIFFRACTION (1.78 Å)